Inflammation and Resolution Are Associated with Upregulation of Fatty Acid β-Oxidation in Zymosan-Induced Peritonitis

Yusuke Fujieda, Atsushi Manno, Yasuhiro Hayashi, Nelson Rhodes, Lining Guo, Makoto Arita, Takeshi Bamba, Eiichiro Fukusaki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Inflammation is a fundamental defensive response to harmful stimuli. However, it can cause damage if it does not subside. To avoid such damage, organisms have developed a mechanism called resolution of inflammation. Here we applied an untargeted metabolomics approach to a sterile and self-resolving animal model of acute inflammation, namely zymosan-induced peritonitis in mice, to examine the effect of inflammation and resolution on the metabolomic profiles. Significant and time-dependent changes in metabolite profiles after zymosan administration were observed in both peritoneal wash fluid (PWF) and plasma. These metabolomic changes correlated well with inflammatory chemokine or cytokine production. In PWF, most of metabolites that could detected increased in zymosan-treated mice, which is suggestive of inflammation, oxidative stress and increased energy demands. In plasma, most metabolites in the central metabolic pathway (glycolysis and TCA cycle) were significantly downregulated after zymosan administration. The concentration of the ketone body 3-hydroxybutyric acid (3-HB) in plasma and PWF increased in zymosan-injected animals indicating upregulation of fatty acid β-oxidation. Increased 3-HB level was observed in the cells that infiltrated into the peritoneal cavity and these infiltrated cells might contribute, at least in part, to the production of 3-HB in the peritoneal cavity.

Original languageEnglish
Article numbere66270
JournalPloS one
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 11 2013
Externally publishedYes

Fingerprint

zymosan
Zymosan
beta oxidation
peritonitis
Peritonitis
3-hydroxybutyric acid
Up-Regulation
Fatty Acids
inflammation
Metabolomics
3-Hydroxybutyric Acid
Ascitic Fluid
Inflammation
metabolomics
Oxidation
Metabolites
Peritoneal Cavity
metabolites
Plasmas
Fluids

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Fujieda, Y., Manno, A., Hayashi, Y., Rhodes, N., Guo, L., Arita, M., ... Fukusaki, E. (2013). Inflammation and Resolution Are Associated with Upregulation of Fatty Acid β-Oxidation in Zymosan-Induced Peritonitis. PloS one, 8(6), [e66270]. https://doi.org/10.1371/journal.pone.0066270

Inflammation and Resolution Are Associated with Upregulation of Fatty Acid β-Oxidation in Zymosan-Induced Peritonitis. / Fujieda, Yusuke; Manno, Atsushi; Hayashi, Yasuhiro; Rhodes, Nelson; Guo, Lining; Arita, Makoto; Bamba, Takeshi; Fukusaki, Eiichiro.

In: PloS one, Vol. 8, No. 6, e66270, 11.06.2013.

Research output: Contribution to journalArticle

Fujieda, Yusuke ; Manno, Atsushi ; Hayashi, Yasuhiro ; Rhodes, Nelson ; Guo, Lining ; Arita, Makoto ; Bamba, Takeshi ; Fukusaki, Eiichiro. / Inflammation and Resolution Are Associated with Upregulation of Fatty Acid β-Oxidation in Zymosan-Induced Peritonitis. In: PloS one. 2013 ; Vol. 8, No. 6.
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