TY - JOUR
T1 - Inflammation-related signaling pathways implicating TGFβ are revealed in the expression profiling of MCF7 cell treated with fermented soybean, Chungkookjang
AU - Hwang, Jae Sung
AU - Yoo, Hyung Jae
AU - Song, Hye Jung
AU - Kim, Kyu Kwang
AU - Chun, Young Jin
AU - Matsui, Toshiro
AU - Kim, Han Bok
N1 - Funding Information:
HB Kim designed the overall research plan and wrote this article. JS Hwang, HJ Yoo, HJ Song, KK Kwang, and YJ Chun conducted research, and HJ Song performed statistical analysis. HB Kim had the primary responsibility for the final content. This work was supported by a grant from National Research Foundation of Korea (2010-0021179).
PY - 2011/5
Y1 - 2011/5
N2 - Chungkookjang is a Korean fermented soybean containing microorganisms, proteinase, and diverse bioactive compounds, including a high concentration of isoflavones and peptides. Growth of breast cancer MCF7 cells decreased dependent on the concentration of fermented soybean extracts. The effect of fermented soybean on cellular gene expression was determined in a systematic manner comprehensively. DNA microarray analysis was performed using 25,804 probes. Ninety one genes whose expression levels were significantly changed were selected. TGFβI and Smad3 were upregulated. Downregulation of inflammation-related CSF2, CSF2RA, and CSF3 was found. Differential expression of chemokines CCL2, CCL3, CCL3L3, CXCL1, and CXCL2 were observed. Network analysis identified ERβ in the network. Based on the experimental results, taking fermented soybean might be helpful for preventing breast cancer by a mechanism activating TGFβ pathway and depressing inflammation.[Supplemental materials are available for this article. Go to the publisher's online edition of Nutrition and Cancer for the following free supplemental resources: Detailed table showing the full list of genes and their analysis details.]
AB - Chungkookjang is a Korean fermented soybean containing microorganisms, proteinase, and diverse bioactive compounds, including a high concentration of isoflavones and peptides. Growth of breast cancer MCF7 cells decreased dependent on the concentration of fermented soybean extracts. The effect of fermented soybean on cellular gene expression was determined in a systematic manner comprehensively. DNA microarray analysis was performed using 25,804 probes. Ninety one genes whose expression levels were significantly changed were selected. TGFβI and Smad3 were upregulated. Downregulation of inflammation-related CSF2, CSF2RA, and CSF3 was found. Differential expression of chemokines CCL2, CCL3, CCL3L3, CXCL1, and CXCL2 were observed. Network analysis identified ERβ in the network. Based on the experimental results, taking fermented soybean might be helpful for preventing breast cancer by a mechanism activating TGFβ pathway and depressing inflammation.[Supplemental materials are available for this article. Go to the publisher's online edition of Nutrition and Cancer for the following free supplemental resources: Detailed table showing the full list of genes and their analysis details.]
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U2 - 10.1080/01635581.2011.551987
DO - 10.1080/01635581.2011.551987
M3 - Article
C2 - 21547849
AN - SCOPUS:79957618657
VL - 63
SP - 645
EP - 652
JO - Nutrition and Cancer
JF - Nutrition and Cancer
SN - 0163-5581
IS - 4
ER -
