Inflammatory platelet production stimulated by tyrosyl-tRNA synthetase mimicking viral infection

Yosuke Morodomi, Sachiko Kanaji, Brian M. Sullivan, Alessandro Zarpellon, Jennifer N. Orje, Eric Won, Ryan Shapiro, Xiang Lei Yang, Wolfram Ruf, Paul Schimmel, Zaverio M. Ruggeri, Taisuke Kanaji

Research output: Contribution to journalArticlepeer-review

Abstract

Platelets play a role not only in hemostasis and thrombosis, but also in inflammation and innate immunity. We previously reported that an activated form of tyrosyl-tRNA synthetase (YRSACT) has an extratranslational activity that enhances megakaryopoiesis and platelet production in mice. Here, we report that YRSACT mimics inflammatory stress inducing a unique megakaryocyte (MK) population with stem cell (Sca1) and myeloid (F4/80) markers through a mechanism dependent on Toll-like receptor (TLR) activation and type I interferon (IFN-I) signaling. This mimicry of inflammatory stress by YRSACT was studied in mice infected by lymphocytic choriomeningitis virus (LCMV). Using Sca1/EGFP transgenic mice, we demonstrated that IFN-I induced by YRSACT or LCMV infection suppressed normal hematopoiesis while activating an alternative pathway of thrombopoiesis. Platelets of inflammatory origin (Sca1/EGFP+) were a relevant proportion of those circulating during recovery from thrombocytopenia. Analysis of these “inflammatory” MKs and platelets suggested their origin in myeloid/MK-biased hematopoietic stem cells (HSCs) that bypassed the classical MK-erythroid progenitor (MEP) pathway to replenish platelets and promote recovery from thrombocytopenia. Notably, inflammatory platelets displayed enhanced agonist-induced activation and procoagulant activities. Moreover, myeloid/MK-biased progenitors and MKs were mobilized from the bone marrow, as evidenced by their presence in the lung microvasculature within fibrin-containing microthrombi. Our results define the function of YRSACT in platelet generation and contribute to elucidate platelet alterations in number and function during viral infection.

Original languageEnglish
Article numbere2212659119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number48
DOIs
Publication statusPublished - Nov 29 2022
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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