Inflammatory stimuli accelerate Sjögren's syndrome-like disease in (NZB x NZW)F1 mice

Umesh S. Deshmukh, Yukiko Ohyama, Harini Bagavant, Xiaoti Guo, Felicia Gaskin, Shu Man Fu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective. This study was undertaken to determine whether induction of systemic inflammation accelerates the development of Sjögren's syndrome (SS) in genetically susceptible mice. Methods. Female (NZB x NZW)F1 mice were treated with either Freund's incomplete adjuvant (IFA) or phosphate buffered saline (PBS) at monthly intervals. Salivary gland function was monitored by measuring pilocarpine-induced saliva volume. Mice were killed at different time points and examined for sialadenitis and salivary gland-infiltrating cells. Sera were analyzed for autoantibodies to salivary gland antigens, nuclear antigens, and Ro60. Results. While IFA-treated mice had significantly decreased salivary secretion 7 weeks after the initial treatment, salivary secretion did not decrease in PBS-treated controls until 17 weeks. At 7 weeks, the severity of sialadenitis and the number of T and B cells infiltrating the salivary glands did not differ between the 2 groups. However, at this time point IFA-treated mice showed significantly higher frequencies of CD11clow, B220+, Ly6C+, mouse PDCA-1+ dendritic cells (DCs) in the salivary glands. While levels of autoantibodies did not differ between the 2 groups at early time points, by late time points IFA-treated mice had higher levels. The gland dysfunction observed in IFA-treated mice at earlier time points did not correlate with the severity of sialadenitis or levels of autoantibodies. Instead, it was associated with increased frequency of plasmacytoid DCs in the gland. Conclusion. Our data suggest that generalized inflammatory stimuli can accelerate the development of SS-like disease in (NZB x NZW)F1 mice, and that gland dysfunction in SS can develop prior to the generation of a robust adaptive autoimmune response.

Original languageEnglish
Pages (from-to)1318-1323
Number of pages6
JournalArthritis and rheumatism
Volume58
Issue number5
DOIs
Publication statusPublished - May 1 2008

Fingerprint

Salivary Glands
Sialadenitis
Autoantibodies
Dendritic Cells
Phosphates
Nuclear Antigens
Pilocarpine
Autoimmunity
Saliva
B-Lymphocytes
Inflammation
T-Lymphocytes
Serum
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Inflammatory stimuli accelerate Sjögren's syndrome-like disease in (NZB x NZW)F1 mice. / Deshmukh, Umesh S.; Ohyama, Yukiko; Bagavant, Harini; Guo, Xiaoti; Gaskin, Felicia; Fu, Shu Man.

In: Arthritis and rheumatism, Vol. 58, No. 5, 01.05.2008, p. 1318-1323.

Research output: Contribution to journalArticle

Deshmukh, Umesh S. ; Ohyama, Yukiko ; Bagavant, Harini ; Guo, Xiaoti ; Gaskin, Felicia ; Fu, Shu Man. / Inflammatory stimuli accelerate Sjögren's syndrome-like disease in (NZB x NZW)F1 mice. In: Arthritis and rheumatism. 2008 ; Vol. 58, No. 5. pp. 1318-1323.
@article{564bc398b5c142ff83a0bb305bd56d3f,
title = "Inflammatory stimuli accelerate Sj{\"o}gren's syndrome-like disease in (NZB x NZW)F1 mice",
abstract = "Objective. This study was undertaken to determine whether induction of systemic inflammation accelerates the development of Sj{\"o}gren's syndrome (SS) in genetically susceptible mice. Methods. Female (NZB x NZW)F1 mice were treated with either Freund's incomplete adjuvant (IFA) or phosphate buffered saline (PBS) at monthly intervals. Salivary gland function was monitored by measuring pilocarpine-induced saliva volume. Mice were killed at different time points and examined for sialadenitis and salivary gland-infiltrating cells. Sera were analyzed for autoantibodies to salivary gland antigens, nuclear antigens, and Ro60. Results. While IFA-treated mice had significantly decreased salivary secretion 7 weeks after the initial treatment, salivary secretion did not decrease in PBS-treated controls until 17 weeks. At 7 weeks, the severity of sialadenitis and the number of T and B cells infiltrating the salivary glands did not differ between the 2 groups. However, at this time point IFA-treated mice showed significantly higher frequencies of CD11clow, B220+, Ly6C+, mouse PDCA-1+ dendritic cells (DCs) in the salivary glands. While levels of autoantibodies did not differ between the 2 groups at early time points, by late time points IFA-treated mice had higher levels. The gland dysfunction observed in IFA-treated mice at earlier time points did not correlate with the severity of sialadenitis or levels of autoantibodies. Instead, it was associated with increased frequency of plasmacytoid DCs in the gland. Conclusion. Our data suggest that generalized inflammatory stimuli can accelerate the development of SS-like disease in (NZB x NZW)F1 mice, and that gland dysfunction in SS can develop prior to the generation of a robust adaptive autoimmune response.",
author = "Deshmukh, {Umesh S.} and Yukiko Ohyama and Harini Bagavant and Xiaoti Guo and Felicia Gaskin and Fu, {Shu Man}",
year = "2008",
month = "5",
day = "1",
doi = "10.1002/art.23368",
language = "English",
volume = "58",
pages = "1318--1323",
journal = "Arthritis and Rheumatology",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Inflammatory stimuli accelerate Sjögren's syndrome-like disease in (NZB x NZW)F1 mice

AU - Deshmukh, Umesh S.

AU - Ohyama, Yukiko

AU - Bagavant, Harini

AU - Guo, Xiaoti

AU - Gaskin, Felicia

AU - Fu, Shu Man

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Objective. This study was undertaken to determine whether induction of systemic inflammation accelerates the development of Sjögren's syndrome (SS) in genetically susceptible mice. Methods. Female (NZB x NZW)F1 mice were treated with either Freund's incomplete adjuvant (IFA) or phosphate buffered saline (PBS) at monthly intervals. Salivary gland function was monitored by measuring pilocarpine-induced saliva volume. Mice were killed at different time points and examined for sialadenitis and salivary gland-infiltrating cells. Sera were analyzed for autoantibodies to salivary gland antigens, nuclear antigens, and Ro60. Results. While IFA-treated mice had significantly decreased salivary secretion 7 weeks after the initial treatment, salivary secretion did not decrease in PBS-treated controls until 17 weeks. At 7 weeks, the severity of sialadenitis and the number of T and B cells infiltrating the salivary glands did not differ between the 2 groups. However, at this time point IFA-treated mice showed significantly higher frequencies of CD11clow, B220+, Ly6C+, mouse PDCA-1+ dendritic cells (DCs) in the salivary glands. While levels of autoantibodies did not differ between the 2 groups at early time points, by late time points IFA-treated mice had higher levels. The gland dysfunction observed in IFA-treated mice at earlier time points did not correlate with the severity of sialadenitis or levels of autoantibodies. Instead, it was associated with increased frequency of plasmacytoid DCs in the gland. Conclusion. Our data suggest that generalized inflammatory stimuli can accelerate the development of SS-like disease in (NZB x NZW)F1 mice, and that gland dysfunction in SS can develop prior to the generation of a robust adaptive autoimmune response.

AB - Objective. This study was undertaken to determine whether induction of systemic inflammation accelerates the development of Sjögren's syndrome (SS) in genetically susceptible mice. Methods. Female (NZB x NZW)F1 mice were treated with either Freund's incomplete adjuvant (IFA) or phosphate buffered saline (PBS) at monthly intervals. Salivary gland function was monitored by measuring pilocarpine-induced saliva volume. Mice were killed at different time points and examined for sialadenitis and salivary gland-infiltrating cells. Sera were analyzed for autoantibodies to salivary gland antigens, nuclear antigens, and Ro60. Results. While IFA-treated mice had significantly decreased salivary secretion 7 weeks after the initial treatment, salivary secretion did not decrease in PBS-treated controls until 17 weeks. At 7 weeks, the severity of sialadenitis and the number of T and B cells infiltrating the salivary glands did not differ between the 2 groups. However, at this time point IFA-treated mice showed significantly higher frequencies of CD11clow, B220+, Ly6C+, mouse PDCA-1+ dendritic cells (DCs) in the salivary glands. While levels of autoantibodies did not differ between the 2 groups at early time points, by late time points IFA-treated mice had higher levels. The gland dysfunction observed in IFA-treated mice at earlier time points did not correlate with the severity of sialadenitis or levels of autoantibodies. Instead, it was associated with increased frequency of plasmacytoid DCs in the gland. Conclusion. Our data suggest that generalized inflammatory stimuli can accelerate the development of SS-like disease in (NZB x NZW)F1 mice, and that gland dysfunction in SS can develop prior to the generation of a robust adaptive autoimmune response.

UR - http://www.scopus.com/inward/record.url?scp=43949096920&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43949096920&partnerID=8YFLogxK

U2 - 10.1002/art.23368

DO - 10.1002/art.23368

M3 - Article

VL - 58

SP - 1318

EP - 1323

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

IS - 5

ER -