Infliximab induces potent anti-inflammatory responses by outside-to-inside signals through transmembrane TNF-α

Hiroki Mitoma, Takahiko Horiuchi, Nobuaki Hatta, Hiroshi Tsukamoto, Shin Ichi Harashima, Yuji Kikuchi, Junji Otsuka, Seiichi Okamura, Shigeru Fujita, Mine Harada

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226 Citations (Scopus)

Abstract

Background & Aims: Both infliximab (chimeric anti-tumor necrosis factor [TNF]-α antibody) and etanercept (p75 TNF-α receptor/immunoglobulin G fusion protein) are effective against rheumatoid arthritis, but only infliximab induces clinical remission in Crohn's disease. To clarify this difference in clinical efficacy, we investigated reverse signaling through transmembrane TNF-α (mTNF) by these 2 anti-TNF agents. Methods: We stably transfected wild-type and cytoplasmic serine-replaced mutant forms of mTNF in human Jurkat T cells. Cells were stimulated with infliximab and etanercept and then analyzed for E-selectin expression, reactive oxygen species accumulation, apoptosis, and cell cycle distribution by flow cytometry. Interleukin-10 and interferon-γ were measured by enzyme-linked immunosorbent assay. Phospho-c-Jun NH2-terminal kinase, Bax, Bak, p21WAF1/CIP1, caspase-8, and caspase-3 were examined by immunoblotting. Results: Both anti-TNF agents induced E-selectin expression, but only infliximab induced interleukin-10 production, apoptosis, and G0/G1 cell cycle arrest. Apoptosis and cell cycle arrest were abolished by substitution of all 3 cytoplasmic serine residues of mTNF by alanine residues. Infliximab induced accumulation of reactive oxygen species and up-regulation of Bax, Bak, and p21WAF1/CIP1 proteins, suggesting the involvement of p53 activation. Moreover, phosphorylation of c-Jun NH2-terminal kinase was necessary for infliximab-induced apoptosis and cell cycle arrest. Conclusions: We revealed the mTNF motifs and the downstream intracellular molecular events essential for reverse signaling through mTNF. The biologic effects of mTNF elicited by infliximab should be important action mechanisms of this potent anti-inflammatory agent in addition to the neutralization of soluble TNF-α. These observations will provide insight into the novel role of mTNF in inflammation.

Original languageEnglish
Pages (from-to)376-392
Number of pages17
JournalGastroenterology
Volume128
Issue number2
DOIs
Publication statusPublished - Feb 2005

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

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    Mitoma, H., Horiuchi, T., Hatta, N., Tsukamoto, H., Harashima, S. I., Kikuchi, Y., Otsuka, J., Okamura, S., Fujita, S., & Harada, M. (2005). Infliximab induces potent anti-inflammatory responses by outside-to-inside signals through transmembrane TNF-α. Gastroenterology, 128(2), 376-392. https://doi.org/10.1053/j.gastro.2004.11.060