Influence of donor age on the cytotoxicity and mutagenicity of ethylnitrosourea in cultured human T-lymphocytes

Nan Mei, Naoki Kunugita, Satoshi Nomoto, Akira Ootsuyama, Toshiyuki Norimura

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of age on the cytotoxicity and mutagenicity of N-ethyl-N- nitrosourea (ENU) in human peripheral blood T-lymphocytes were investigated using colony-forming assay in vitro. ENU was shown to induce a dose-dependent increase in cell killing and in mutation frequencies (MF). No significant correlation between age and ENU induced 6-thioguanine-resistant (TG(r)) MF at the hypoxanthine phosphoribosyl transferase (HPRT) locus of the X-chromosome was found after treatment with the same concentration of ENU (1 mM or 2 mM). There were also no significant differences among different donor age groups and the sensitivity parameters for exposure to ENU. As X-rays, the cytotoxic and mutagenic effects of ENU in cultured human T-lymphocytes appear not to be associated with age. These results suggest that the repair of mutagen- induced DNA lesions does not decline with age. Such knowledge has implications for risk assessment and protection against environmental mutagens.

Original languageEnglish
Pages (from-to)133-145
Number of pages13
JournalJournal of UOEH
Volume19
Issue number2
DOIs
Publication statusPublished - Jun 1 1997

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

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