Influence of dosing time on pharmacological action of G-CSF in mice

Shigehiro Ohdo, Taku Furukubo, Naoko Arata, Eiji Yukawa, Shun Higuchi, Shigeyuki Nakano, Nobuya Ogawa

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10 Citations (Scopus)

Abstract

The role of the susceptibility of living organisms and the pharmacokinetics of G-CSF on the rhythm of granulocyte colony-stimulating factor (G-CSF) activity was investigated. ICR male mice were housed in a standardized light-dark cycle (lights on at 0700, off at 1900) with food and water ad libitum. The leukocyte counts at 2 and 24 hr after G-CSF (250 μg/kg, iv) injection were significantly higher in mice injected with the drug at 0700 than at 1900 (p < 0.01, respectively). The higher leukocyte-increasing effect corresponded to drug dosing at the time in which the granulocyte colony formation stimulated by G-CSF and DNA synthesis increased and the lower effect corresponded to drug dosing at the time in which they decreased. The rhythmicity corresponded to that in plasma G-CSF concentration. The present study suggests that the rhythm of G-CSF activity is caused by that of the sensitivity of bone marrow cells to the drug and the pharmacokinetics of the drug.

Original languageEnglish
Pages (from-to)PL163-PL168
JournalLife Sciences
Volume62
Issue number12
DOIs
Publication statusPublished - Feb 13 1998

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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