Influence of groove depth and surface profile on fluorescence enhancement by grating-coupled surface plasmon resonance

Hironobu Hori; Keiko Tawa; Kenji Kintaka; Junji Nishii; Yoshiro Tatsu

doi:10.1007/s10043-009-0040-5

Influence of groove depth and surface profile on fluorescence enhancement by grating-coupled surface plasmon resonance

Hironobu Hori, Keiko Tawa, Kenji Kintaka, Junji Nishii, Yoshiro Tatsu

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The fluorescence intensity of a sample placed on a metal grating pattern is enhanced due to excitation by the electric field of the grating-coupled surface plasmon resonance (GC-SPR). The dependence of the enhancement on groove depth and surface profile was studied with the aim of improving the sensitivity of fluorescence detection. The enhancement was found to depend on the groove depth, with intensity most enhanced on grating substrate of about 20 nm depth, which produced an intensity about 30 times greater than that on a flat borosilicate glass substrate. Rigorous coupled wave analysis calculation showed that the shape of the groove influenced GC-SPR, suggesting that controlling not only the depth but also the shape of the grating surface profile can be an important factor in improving the sensitivity of detection by fluorescence microscopy.

Original language	English
Pages (from-to)	216-221
Number of pages	6
Journal	Optical Review
Volume	16
Issue number	2
DOIs	https://doi.org/10.1007/s10043-009-0040-5
Publication status	Published - Mar 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1007/s10043-009-0040-5

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title = "Influence of groove depth and surface profile on fluorescence enhancement by grating-coupled surface plasmon resonance",

abstract = "The fluorescence intensity of a sample placed on a metal grating pattern is enhanced due to excitation by the electric field of the grating-coupled surface plasmon resonance (GC-SPR). The dependence of the enhancement on groove depth and surface profile was studied with the aim of improving the sensitivity of fluorescence detection. The enhancement was found to depend on the groove depth, with intensity most enhanced on grating substrate of about 20 nm depth, which produced an intensity about 30 times greater than that on a flat borosilicate glass substrate. Rigorous coupled wave analysis calculation showed that the shape of the groove influenced GC-SPR, suggesting that controlling not only the depth but also the shape of the grating surface profile can be an important factor in improving the sensitivity of detection by fluorescence microscopy.",

author = "Hironobu Hori and Keiko Tawa and Kenji Kintaka and Junji Nishii and Yoshiro Tatsu",

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T1 - Influence of groove depth and surface profile on fluorescence enhancement by grating-coupled surface plasmon resonance

AU - Hori, Hironobu

AU - Tawa, Keiko

AU - Kintaka, Kenji

AU - Nishii, Junji

AU - Tatsu, Yoshiro

PY - 2009/3

Y1 - 2009/3

N2 - The fluorescence intensity of a sample placed on a metal grating pattern is enhanced due to excitation by the electric field of the grating-coupled surface plasmon resonance (GC-SPR). The dependence of the enhancement on groove depth and surface profile was studied with the aim of improving the sensitivity of fluorescence detection. The enhancement was found to depend on the groove depth, with intensity most enhanced on grating substrate of about 20 nm depth, which produced an intensity about 30 times greater than that on a flat borosilicate glass substrate. Rigorous coupled wave analysis calculation showed that the shape of the groove influenced GC-SPR, suggesting that controlling not only the depth but also the shape of the grating surface profile can be an important factor in improving the sensitivity of detection by fluorescence microscopy.

AB - The fluorescence intensity of a sample placed on a metal grating pattern is enhanced due to excitation by the electric field of the grating-coupled surface plasmon resonance (GC-SPR). The dependence of the enhancement on groove depth and surface profile was studied with the aim of improving the sensitivity of fluorescence detection. The enhancement was found to depend on the groove depth, with intensity most enhanced on grating substrate of about 20 nm depth, which produced an intensity about 30 times greater than that on a flat borosilicate glass substrate. Rigorous coupled wave analysis calculation showed that the shape of the groove influenced GC-SPR, suggesting that controlling not only the depth but also the shape of the grating surface profile can be an important factor in improving the sensitivity of detection by fluorescence microscopy.

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JO - Optical Review

JF - Optical Review

SN - 1340-6000

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Influence of groove depth and surface profile on fluorescence enhancement by grating-coupled surface plasmon resonance

Abstract

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