Influence of ionic liquid and ionic salt on protein against the reactive species generated using dielectric barrier discharge plasma

Pankaj Attri, Thapanut Sarinont, Minsup Kim, Takaaki Amano, Kazunori Koga, Art E. Cho, Eun Ha Choi, Masaharu Shiratani

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The presence of salts in biological solution can affect the activity of the reactive species (RS) generated by plasma, and so they can also have an influence on the plasma-induced sterilization. In this work, we assess the influence that diethylammonium dihydrogen phosphate (DEAP), an ionic liquid (IL), and sodium chloride (NaCl), an ionic salt (IS), have on the structural changes in hemoglobin (Hb) in the presence of RS generated using dielectric barrier discharge (DBD) plasma in the presence of various gases [O2, N2, Ar, He, NO (10%) + N2 and Air]. We carry out fluorescence spectroscopy to verify the generation of •OH with or without the presence of DEAP IL and IS, and we use electron spin resonance (ESR) to check the generation of H • and • OH. In addition, we verified the structural changes in the Hb structure after treatment with DBD in presence and absence of IL and IS. We then assessed the structural stability of the Hb in the presence of IL and IS by using molecular dynamic (MD) simulations. Our results indicate that the IL has a strong effect on the conservation of the Hb structure relative to that of IS against RS generated by plasma.

Original languageEnglish
Article number17781
JournalScientific reports
Volume5
DOIs
Publication statusPublished - Dec 10 2015

Fingerprint

Ionic Liquids
Salts
Hemoglobins
Proteins
Phosphates
Fluorescence Spectrometry
Electron Spin Resonance Spectroscopy
Molecular Dynamics Simulation
Sodium Chloride
Gases
Air

All Science Journal Classification (ASJC) codes

  • General

Cite this

Influence of ionic liquid and ionic salt on protein against the reactive species generated using dielectric barrier discharge plasma. / Attri, Pankaj; Sarinont, Thapanut; Kim, Minsup; Amano, Takaaki; Koga, Kazunori; Cho, Art E.; Choi, Eun Ha; Shiratani, Masaharu.

In: Scientific reports, Vol. 5, 17781, 10.12.2015.

Research output: Contribution to journalArticle

@article{5e5467afa8334fb0aed4cd6cd01c7bd3,
title = "Influence of ionic liquid and ionic salt on protein against the reactive species generated using dielectric barrier discharge plasma",
abstract = "The presence of salts in biological solution can affect the activity of the reactive species (RS) generated by plasma, and so they can also have an influence on the plasma-induced sterilization. In this work, we assess the influence that diethylammonium dihydrogen phosphate (DEAP), an ionic liquid (IL), and sodium chloride (NaCl), an ionic salt (IS), have on the structural changes in hemoglobin (Hb) in the presence of RS generated using dielectric barrier discharge (DBD) plasma in the presence of various gases [O2, N2, Ar, He, NO (10{\%}) + N2 and Air]. We carry out fluorescence spectroscopy to verify the generation of •OH with or without the presence of DEAP IL and IS, and we use electron spin resonance (ESR) to check the generation of H • and • OH. In addition, we verified the structural changes in the Hb structure after treatment with DBD in presence and absence of IL and IS. We then assessed the structural stability of the Hb in the presence of IL and IS by using molecular dynamic (MD) simulations. Our results indicate that the IL has a strong effect on the conservation of the Hb structure relative to that of IS against RS generated by plasma.",
author = "Pankaj Attri and Thapanut Sarinont and Minsup Kim and Takaaki Amano and Kazunori Koga and Cho, {Art E.} and Choi, {Eun Ha} and Masaharu Shiratani",
year = "2015",
month = "12",
day = "10",
doi = "10.1038/srep17781",
language = "English",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Influence of ionic liquid and ionic salt on protein against the reactive species generated using dielectric barrier discharge plasma

AU - Attri, Pankaj

AU - Sarinont, Thapanut

AU - Kim, Minsup

AU - Amano, Takaaki

AU - Koga, Kazunori

AU - Cho, Art E.

AU - Choi, Eun Ha

AU - Shiratani, Masaharu

PY - 2015/12/10

Y1 - 2015/12/10

N2 - The presence of salts in biological solution can affect the activity of the reactive species (RS) generated by plasma, and so they can also have an influence on the plasma-induced sterilization. In this work, we assess the influence that diethylammonium dihydrogen phosphate (DEAP), an ionic liquid (IL), and sodium chloride (NaCl), an ionic salt (IS), have on the structural changes in hemoglobin (Hb) in the presence of RS generated using dielectric barrier discharge (DBD) plasma in the presence of various gases [O2, N2, Ar, He, NO (10%) + N2 and Air]. We carry out fluorescence spectroscopy to verify the generation of •OH with or without the presence of DEAP IL and IS, and we use electron spin resonance (ESR) to check the generation of H • and • OH. In addition, we verified the structural changes in the Hb structure after treatment with DBD in presence and absence of IL and IS. We then assessed the structural stability of the Hb in the presence of IL and IS by using molecular dynamic (MD) simulations. Our results indicate that the IL has a strong effect on the conservation of the Hb structure relative to that of IS against RS generated by plasma.

AB - The presence of salts in biological solution can affect the activity of the reactive species (RS) generated by plasma, and so they can also have an influence on the plasma-induced sterilization. In this work, we assess the influence that diethylammonium dihydrogen phosphate (DEAP), an ionic liquid (IL), and sodium chloride (NaCl), an ionic salt (IS), have on the structural changes in hemoglobin (Hb) in the presence of RS generated using dielectric barrier discharge (DBD) plasma in the presence of various gases [O2, N2, Ar, He, NO (10%) + N2 and Air]. We carry out fluorescence spectroscopy to verify the generation of •OH with or without the presence of DEAP IL and IS, and we use electron spin resonance (ESR) to check the generation of H • and • OH. In addition, we verified the structural changes in the Hb structure after treatment with DBD in presence and absence of IL and IS. We then assessed the structural stability of the Hb in the presence of IL and IS by using molecular dynamic (MD) simulations. Our results indicate that the IL has a strong effect on the conservation of the Hb structure relative to that of IS against RS generated by plasma.

UR - http://www.scopus.com/inward/record.url?scp=84949579878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949579878&partnerID=8YFLogxK

U2 - 10.1038/srep17781

DO - 10.1038/srep17781

M3 - Article

VL - 5

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 17781

ER -