Influence of meiotic inhibition by butyrolactone-I during germinal vesicle stage on the ability of porcine oocytes to be activated by electric stimulation after nuclear maturation

Yuji Hirao, Namiko Nishimoto, Satoshi Kure-bayashi, Naoki Takenouchi, Nobuhiko Yamauchi, Hiroshi Masuda, Takashi Nagai

Research output: Contribution to journalArticle

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Abstract

Butyrolactone-I (BL-I) is a specific inhibitor of cyclin-dependent kinases and prevents germinal vesicle breakdown (GVBD) in porcine oocytes. This study first focused on the effect of BL-I on the time course of GVBD and progression to metaphase II (MII) in oocytes after the removal of BL-I. When porcine oocytes were treated with 20 μM BL-I for 28 h, the intervals taken to undergo GVBD and progress to MII were 4-8 h and 16-20 h after washing out BL-I, respectively. These intervals were both approximately 8 h behind those of the control oocytes. When the BL-I treatment was performed for 20 h, instead of 28 h, there were no differences in the timing or frequency of progression to Mil between the BL-I treatment and control groups. To determine whether the cytoplasmic maturation was the same, the ability of oocytes to form a female pronucleus in response to an electric stimulus was examined. When oocytes were stimulated at 28 h after the removal of BL-I, the rate of pronucleus formation was significantly lower in oocytes treated with BL-I than in untreated oocytes, despite the fact that both groups reached MII at a similar time. When the electric stimulus was given at 36 h after the removal of BL-I, oocytes had a pronucleus formation rate comparable to that of control oocytes. Therefore, the findings suggest that BL-I treatment of porcine oocytes at the germinal vesicle stage affects not only the progression of meiosis, but also the events involved in cytoplasmic maturation.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalZygote
Volume11
Issue number3
DOIs
Publication statusPublished - Aug 1 2003
Externally publishedYes

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Electric Stimulation
Oocytes
Swine
Metaphase
butyrolactone I
Cyclin-Dependent Kinases
Meiosis
Control Groups

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Influence of meiotic inhibition by butyrolactone-I during germinal vesicle stage on the ability of porcine oocytes to be activated by electric stimulation after nuclear maturation. / Hirao, Yuji; Nishimoto, Namiko; Kure-bayashi, Satoshi; Takenouchi, Naoki; Yamauchi, Nobuhiko; Masuda, Hiroshi; Nagai, Takashi.

In: Zygote, Vol. 11, No. 3, 01.08.2003, p. 191-197.

Research output: Contribution to journalArticle

Hirao, Yuji ; Nishimoto, Namiko ; Kure-bayashi, Satoshi ; Takenouchi, Naoki ; Yamauchi, Nobuhiko ; Masuda, Hiroshi ; Nagai, Takashi. / Influence of meiotic inhibition by butyrolactone-I during germinal vesicle stage on the ability of porcine oocytes to be activated by electric stimulation after nuclear maturation. In: Zygote. 2003 ; Vol. 11, No. 3. pp. 191-197.
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