Influence of menstruation on the microbiota of healthy women's labia minora as analyzed using a 16s rRNA gene-based clone library method

Tsukasa Shiraishi, Kazumasa Fukuda, Nobuo Morotomi, Yuri Imamura, Junko Mishima, Shigeo Imai, Kiyoshi Miyazawa, Hatsumi Taniguchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The aim of this study was to determine the influence of menstruation on the bacterial population of healthy Japanese women's vulvas, especially the labia minora. Labia minora swabs were obtained from 10 premenopausal, nonpregnant Japanese women at premenstruation and on day 2 of menstruation. Vaginal swabs were also obtained from 3 out of the 10 women. No significant difference was found in the average bacterial cell count between the menstruation and premenstruation samples. Molecular analysis using a 16S rRNA gene-based clone library method detected 22 genera from the labia minora swabs (total 20), with the genus Lactobacillus being predominant at both premenstruation and during menstruation in 7 out of the 10 women. Of the other 3 women, 2 showed various kinds of bacterial species, including oral and fecal bacteria, with Atopobium vaginae and Gardnerella vaginalis predominating in the remaining woman's vulva in both conditions. In total, 6 out of 10 cases (60%) showed significantly different microbiota of the labia minora between the two conditions. These results imply that menstruation may promote a distortion of the bacterial flora around the vulva, although it causes no significant increase of the bacterial count.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalJapanese Journal of Infectious Diseases
Volume64
Issue number1
Publication statusPublished - Feb 8 2011
Externally publishedYes

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Menstruation
Microbiota
rRNA Genes
Clone Cells
Vulva
Bacterial Load
Gardnerella vaginalis
Lactobacillus
Vagina
Cell Count
Bacteria
Population

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Influence of menstruation on the microbiota of healthy women's labia minora as analyzed using a 16s rRNA gene-based clone library method. / Shiraishi, Tsukasa; Fukuda, Kazumasa; Morotomi, Nobuo; Imamura, Yuri; Mishima, Junko; Imai, Shigeo; Miyazawa, Kiyoshi; Taniguchi, Hatsumi.

In: Japanese Journal of Infectious Diseases, Vol. 64, No. 1, 08.02.2011, p. 76-80.

Research output: Contribution to journalArticle

Shiraishi, Tsukasa ; Fukuda, Kazumasa ; Morotomi, Nobuo ; Imamura, Yuri ; Mishima, Junko ; Imai, Shigeo ; Miyazawa, Kiyoshi ; Taniguchi, Hatsumi. / Influence of menstruation on the microbiota of healthy women's labia minora as analyzed using a 16s rRNA gene-based clone library method. In: Japanese Journal of Infectious Diseases. 2011 ; Vol. 64, No. 1. pp. 76-80.
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