Influence of N-methyl-N-nitrosourea treatment on embryogenesis of Bombyx mori

Yutaka Kawaguchi, Takahiro Kusakabe, Katsumi Koga

Research output: Contribution to journalArticlepeer-review

Abstract

Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5 h after oviposition in a 10 mM N-methyl-N-nitrosourea (MNU) solution for 15 min. About 80% of thus treated eggs could not hatch. When observed under a light microscope using unsectioned transparent specimens of unhatched eggs, the embryos exhibited abnormalities such as the absence of inner organs, twisting body axis, incomplete body segments and anomalous segmentation. These findings confirmed that the immersing method worked well and MNU strongly provokes various forms of somatic impairment in developing embryos.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume48
Issue number1-2
Publication statusPublished - Oct 1 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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