Abstract
Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5 h after oviposition in a 10 mM N-methyl-N-nitrosourea (MNU) solution for 15 min. About 80% of thus treated eggs could not hatch. When observed under a light microscope using unsectioned transparent specimens of unhatched eggs, the embryos exhibited abnormalities such as the absence of inner organs, twisting body axis, incomplete body segments and anomalous segmentation. These findings confirmed that the immersing method worked well and MNU strongly provokes various forms of somatic impairment in developing embryos.
| Original language | English |
|---|---|
| Pages (from-to) | 59-64 |
| Number of pages | 6 |
| Journal | Journal of the Faculty of Agriculture, Kyushu University |
| Volume | 48 |
| Issue number | 1-2 |
| Publication status | Published - Oct 1 2003 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Agronomy and Crop Science
Cite this
Influence of N-methyl-N-nitrosourea treatment on embryogenesis of Bombyx mori. / Kawaguchi, Yutaka; Kusakabe, Takahiro; Koga, Katsumi.
In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 48, No. 1-2, 01.10.2003, p. 59-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of N-methyl-N-nitrosourea treatment on embryogenesis of Bombyx mori
AU - Kawaguchi, Yutaka
AU - Kusakabe, Takahiro
AU - Koga, Katsumi
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5 h after oviposition in a 10 mM N-methyl-N-nitrosourea (MNU) solution for 15 min. About 80% of thus treated eggs could not hatch. When observed under a light microscope using unsectioned transparent specimens of unhatched eggs, the embryos exhibited abnormalities such as the absence of inner organs, twisting body axis, incomplete body segments and anomalous segmentation. These findings confirmed that the immersing method worked well and MNU strongly provokes various forms of somatic impairment in developing embryos.
AB - Embryonic malformation was induced by immersing Bombyx mori eggs at 3.5 h after oviposition in a 10 mM N-methyl-N-nitrosourea (MNU) solution for 15 min. About 80% of thus treated eggs could not hatch. When observed under a light microscope using unsectioned transparent specimens of unhatched eggs, the embryos exhibited abnormalities such as the absence of inner organs, twisting body axis, incomplete body segments and anomalous segmentation. These findings confirmed that the immersing method worked well and MNU strongly provokes various forms of somatic impairment in developing embryos.
UR - http://www.scopus.com/inward/record.url?scp=1642477590&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1642477590&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:1642477590
VL - 48
SP - 59
EP - 64
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
SN - 0023-6152
IS - 1-2
ER -