TY - JOUR
T1 - Influence of protic ionic liquids on the structure and stability of succinylated Con A
AU - Attri, Pankaj
AU - Venkatesu, Pannuru
N1 - Funding Information:
We gratefully acknowledge the Council of Scientific Industrial Research (CSIR), New Delhi , through the grant No. 01(2343)/09/EMR-II , Department of Science and Technology (DST), New Delhi, India (Grant No. SR/SI/PC-54/2008 ) and University Grants Commission (UGC), New Delhi for financial support and advanced Instrumentation facility centre of Jawaharlal Nehru University, New Delhi for providing CD facility.
PY - 2012/7
Y1 - 2012/7
N2 - We report the synthesis of a series of ionic liquids (ILs) from various ions having different kosmotropicity including dihydrogen phosphate (H2PO4-), hydrogen sulfate (HSO4-) and acetate (CH3COO-) as anions and chaotropic cation such as trialkylammonium cation. To characterize the biomolecular interactions of ILs with protein, we have explored the stability of succinylated Con A (S Con A) in the presence of these aqueous ILs, which are varied combinations of kosmotropic anion with chaotropic cation such as triethylammonium dihydrogen phosphate [(CH3CH2)3NH][H2PO4] (TEAP), trimethylammonium acetate [(CH3)3NH][CH3COO] (TMAA), trimethylammonium dihydrogen phosphate [(CH3)3NH][H2PO4] (TMAP) and trimethylammonium hydrogen sulfate [(CH3)3NH][HSO4] (TMAS). Circular dichroism (CD) and fluorescence experiments have been used to characterize the stability of S Con A by ILs. Our data distinctly demonstrate that the long alkyl chain IL TEAP is a strong stabilizer for S Con A. Further, our experimental results reveal that TEAP is an effective refolding enhancer for S Con A from a thermally denatured protein structure.
AB - We report the synthesis of a series of ionic liquids (ILs) from various ions having different kosmotropicity including dihydrogen phosphate (H2PO4-), hydrogen sulfate (HSO4-) and acetate (CH3COO-) as anions and chaotropic cation such as trialkylammonium cation. To characterize the biomolecular interactions of ILs with protein, we have explored the stability of succinylated Con A (S Con A) in the presence of these aqueous ILs, which are varied combinations of kosmotropic anion with chaotropic cation such as triethylammonium dihydrogen phosphate [(CH3CH2)3NH][H2PO4] (TEAP), trimethylammonium acetate [(CH3)3NH][CH3COO] (TMAA), trimethylammonium dihydrogen phosphate [(CH3)3NH][H2PO4] (TMAP) and trimethylammonium hydrogen sulfate [(CH3)3NH][HSO4] (TMAS). Circular dichroism (CD) and fluorescence experiments have been used to characterize the stability of S Con A by ILs. Our data distinctly demonstrate that the long alkyl chain IL TEAP is a strong stabilizer for S Con A. Further, our experimental results reveal that TEAP is an effective refolding enhancer for S Con A from a thermally denatured protein structure.
UR - http://www.scopus.com/inward/record.url?scp=84861962645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861962645&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2012.04.017
DO - 10.1016/j.ijbiomac.2012.04.017
M3 - Article
C2 - 22542630
AN - SCOPUS:84861962645
VL - 51
SP - 119
EP - 128
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
IS - 1-2
ER -