Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film

Sadaaki Yamamoto, Hiroshi Sunami, Masaru Tanaka, Yasutaka Matsuo, Kuniharu Ijiro, Shigeko Yamashita, Yuka Morita, Masatsugu Shimomura

Research output: Contribution to conferencePaper

Abstract

The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.

Original languageEnglish
Number of pages1
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: May 25 2005May 27 2005

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period5/25/055/27/05

Fingerprint

Surface morphology
Microscopes
Adsorption
Substrates
Adhesives
Adhesion
Proteins
Scanning
Lasers

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Yamamoto, S., Sunami, H., Tanaka, M., Matsuo, Y., Ijiro, K., Yamashita, S., ... Shimomura, M. (2005). Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film. / Yamamoto, Sadaaki; Sunami, Hiroshi; Tanaka, Masaru; Matsuo, Yasutaka; Ijiro, Kuniharu; Yamashita, Shigeko; Morita, Yuka; Shimomura, Masatsugu.

2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Research output: Contribution to conferencePaper

Yamamoto, S, Sunami, H, Tanaka, M, Matsuo, Y, Ijiro, K, Yamashita, S, Morita, Y & Shimomura, M 2005, 'Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film', Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 5/25/05 - 5/27/05.
Yamamoto S, Sunami H, Tanaka M, Matsuo Y, Ijiro K, Yamashita S et al. Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film. 2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.
Yamamoto, Sadaaki ; Sunami, Hiroshi ; Tanaka, Masaru ; Matsuo, Yasutaka ; Ijiro, Kuniharu ; Yamashita, Shigeko ; Morita, Yuka ; Shimomura, Masatsugu. / Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.1 p.
@conference{6c54ba13097d4d47bdcc042862ebd5f4,
title = "Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film",
abstract = "The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.",
author = "Sadaaki Yamamoto and Hiroshi Sunami and Masaru Tanaka and Yasutaka Matsuo and Kuniharu Ijiro and Shigeko Yamashita and Yuka Morita and Masatsugu Shimomura",
year = "2005",
month = "12",
day = "1",
language = "English",
note = "54th SPSJ Annual Meeting 2005 ; Conference date: 25-05-2005 Through 27-05-2005",

}

TY - CONF

T1 - Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film

AU - Yamamoto, Sadaaki

AU - Sunami, Hiroshi

AU - Tanaka, Masaru

AU - Matsuo, Yasutaka

AU - Ijiro, Kuniharu

AU - Yamashita, Shigeko

AU - Morita, Yuka

AU - Shimomura, Masatsugu

PY - 2005/12/1

Y1 - 2005/12/1

N2 - The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.

AB - The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.

UR - http://www.scopus.com/inward/record.url?scp=33645641898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645641898&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645641898

ER -