Influence of the difference in refractive index on the interface of an object and the surroundings in near-infrared fluorescence tomography

Maho Takematsu; Masakazu Umezawa; Toshihiro Sera; Yuichi Kitagawa; Hiroyuki Kurahashi; Shuta Yamada; Kyohei Okubo; Masao Kamimura; Hideo Yokota; Kohei Soga

doi:10.1364/AO.445357

Influence of the difference in refractive index on the interface of an object and the surroundings in near-infrared fluorescence tomography

Maho Takematsu, Masakazu Umezawa, Toshihiro Sera, Yuichi Kitagawa, Hiroyuki Kurahashi, Shuta Yamada, Kyohei Okubo, Masao Kamimura, Hideo Yokota, Kohei Soga

Biochemical Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest fluorescent point is away from the correct point on the tomograms owing to the refraction. The speculated position is determined as the exact point if the RI ratio ranges between 0.97 and 1.03 by immersing the body in an RI matching liquid. The results can help in experimental settings of fluorescence CT for acquiring three-dimensional positional information.

Original language	English
Pages (from-to)	638-644
Number of pages	7
Journal	Applied Optics
Volume	61
Issue number	2
DOIs	https://doi.org/10.1364/AO.445357
Publication status	Published - Jan 10 2022

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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10.1364/AO.445357

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title = "Influence of the difference in refractive index on the interface of an object and the surroundings in near-infrared fluorescence tomography",

abstract = "The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest fluorescent point is away from the correct point on the tomograms owing to the refraction. The speculated position is determined as the exact point if the RI ratio ranges between 0.97 and 1.03 by immersing the body in an RI matching liquid. The results can help in experimental settings of fluorescence CT for acquiring three-dimensional positional information.",

author = "Maho Takematsu and Masakazu Umezawa and Toshihiro Sera and Yuichi Kitagawa and Hiroyuki Kurahashi and Shuta Yamada and Kyohei Okubo and Masao Kamimura and Hideo Yokota and Kohei Soga",

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T1 - Influence of the difference in refractive index on the interface of an object and the surroundings in near-infrared fluorescence tomography

AU - Takematsu, Maho

AU - Umezawa, Masakazu

AU - Sera, Toshihiro

AU - Kitagawa, Yuichi

AU - Kurahashi, Hiroyuki

AU - Yamada, Shuta

AU - Okubo, Kyohei

AU - Kamimura, Masao

AU - Yokota, Hideo

AU - Soga, Kohei

N1 - Funding Information: Funding. Japan Science and Technology Agency (COINS); Ministry of Education, Culture, Sports, Science and Technology (15H05950, 19H01179). Publisher Copyright: © 2022 Optica Publishing Group

PY - 2022/1/10

Y1 - 2022/1/10

N2 - The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest fluorescent point is away from the correct point on the tomograms owing to the refraction. The speculated position is determined as the exact point if the RI ratio ranges between 0.97 and 1.03 by immersing the body in an RI matching liquid. The results can help in experimental settings of fluorescence CT for acquiring three-dimensional positional information.

AB - The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest fluorescent point is away from the correct point on the tomograms owing to the refraction. The speculated position is determined as the exact point if the RI ratio ranges between 0.97 and 1.03 by immersing the body in an RI matching liquid. The results can help in experimental settings of fluorescence CT for acquiring three-dimensional positional information.

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Influence of the difference in refractive index on the interface of an object and the surroundings in near-infrared fluorescence tomography

Abstract

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