TY - JOUR
T1 - Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability
AU - Soria, J. M.
AU - Martínez Ramos, C.
AU - Bahamonde, O.
AU - García Cruz, D. M.
AU - Salmerón Sánchez, M.
AU - García Esparza, M. A.
AU - Casas, C.
AU - Guzmán, M.
AU - Navarro, X.
AU - Gómez Ribelles, J. L.
AU - García Verdugo, J. M.
AU - Monleón Pradas, M.
AU - Barcia, J. A.
PY - 2007/11/1
Y1 - 2007/11/1
N2 - A series of polymeric biomaterials including poly(methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro.
AB - A series of polymeric biomaterials including poly(methyl acrylate) (PMA), chitosan (CHT), poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate and hydroxyethyl acrylate monomeric units were tested in vitro as culture substrates and compared for their impact on the proliferation and expansion of Schwann cells (SCs). Immunocytochemical staining assay and scanning electron microscopy techniques were applied to perform a quantitative analysis to determine the correct maintenance of the cultured glial cells on the different biomaterials. The results strongly suggest that cell attachment and proliferation is influenced by the substrate's surface chemistry, and that hydrophobic biomaterials based on PMA, PEA, and the copolymers PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and proliferation of SCs in vitro.
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U2 - 10.1002/jbm.a.31297
DO - 10.1002/jbm.a.31297
M3 - Article
C2 - 17477391
AN - SCOPUS:35048858444
SN - 1549-3296
VL - 83
SP - 463
EP - 470
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
IS - 2
ER -