Influences of electroosmotic flows in nanopillar chips on DNA separation: Experimental results and numerical simulations

Noritada Kaji, Akio Oki, Ryo Ogawa, Yuzuru Takamura, Takahiro Nishimoto, Hiroaki Nakanishi, Yasuhiro Horiike, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The various potential factors affecting the performance of nanopillar chips on DNA separation were investigated from the viewpoints of both numerical calculations and actual experiments. To yield higher performance and replace the conventional DNA separation techniques such as microchip electrophoresis, the phenomenon specific to the nanopillar chips should be deeply understood. In this paper, although various factors affecting the performance of the nanopillar chips are considered, we focused on the effect of electroosmotic flow, which is particularly noticeable in quartz-made nanopillar chips. High-resolution separation of DNA was realized when an electroosmotic flow was suppressed by simply using a higher concentration of buffer, but DNA separation failed in the presence of an electroosmotic flow. It was confirmed from the numerical simulations and the direct observations that the deformation of DNA band during the injection process was induced by electroosmotic flow and consequently led to a poor resolution.

Original languageEnglish
Pages (from-to)161-169
Number of pages9
JournalIsrael Journal of Chemistry
Volume47
Issue number2
DOIs
Publication statusPublished - Dec 1 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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  • Cite this

    Kaji, N., Oki, A., Ogawa, R., Takamura, Y., Nishimoto, T., Nakanishi, H., Horiike, Y., Tokeshi, M., & Baba, Y. (2007). Influences of electroosmotic flows in nanopillar chips on DNA separation: Experimental results and numerical simulations. Israel Journal of Chemistry, 47(2), 161-169. https://doi.org/10.1560/IJC.47.2.161