Influences of interferon-gamma on cell proliferation and interleukin-6 production in Down syndrome derived fibroblasts

Tsutomu Iwamoto, Aya Yamada, Kenji Yuasa, Emiko Fukumoto, Takashi Nakamura, Taku Fujiwara, Satoshi Fukumoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: Down syndrome, a frequently encountered genetic disorder, is usually associated with medical problems related to infectious disease, such as periodontal diseases and prolonged wound healing. Although affected individuals are considered to have clinical problems related to high interferon (IFN) sensitivity, the molecular mechanisms of IFN activities are not completely understood. Design: Down syndrome derived fibroblasts, Detroit 539 (D1) and Hs 52.Sk (D2) cells, were used. To analyse the expressions of interferon (IFN) receptors and downstream of IFN-γ, western blotting was performed. Cell proliferation was determined by counting cells following trypan blue staining. Media levels of IL-1β, TNF-α, and IL-6 were quantified using ELISA. Results: IFN-γ receptor 2 and IFN-α receptor 1, but not IFN-γ receptor 1, were highly expressed in D1 and D2 cells, as compared to the control fibroblast cells. Cell proliferation by D1 and D2 cells was lower than that by the control fibroblasts, further, IFN-γ had a greater effect to inhibit cell proliferation by D1 and D2 cells. In addition, IFN-γ treatment increased the phosphorylation of STAT1 and MAPK in D1 cells as compared to normal fibroblasts. Also, the presence of exogenous IFN-γ in the growth medium significantly induced IL-6, but not IL-1β or TNF-α, in D1 and D2 cells. Conclusion: Taken together, our results are consistent with hypersensitive reactions to IFN-γ seen in patients with Down syndrome and may provide useful information to elucidate the mechanisms of IFN-γ activities in those individuals.

Original languageEnglish
Pages (from-to)963-969
Number of pages7
JournalArchives of Oral Biology
Volume54
Issue number10
DOIs
Publication statusPublished - Oct 1 2009
Externally publishedYes

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Down Syndrome
Interferon-gamma
Interleukin-6
Interferons
Fibroblasts
Cell Proliferation
Interferon Receptors
Interleukin-1
Inborn Genetic Diseases
Trypan Blue
Interferon-beta
Periodontal Diseases
Wound Healing
Communicable Diseases
Western Blotting
Enzyme-Linked Immunosorbent Assay
Phosphorylation
Staining and Labeling
Growth

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Influences of interferon-gamma on cell proliferation and interleukin-6 production in Down syndrome derived fibroblasts. / Iwamoto, Tsutomu; Yamada, Aya; Yuasa, Kenji; Fukumoto, Emiko; Nakamura, Takashi; Fujiwara, Taku; Fukumoto, Satoshi.

In: Archives of Oral Biology, Vol. 54, No. 10, 01.10.2009, p. 963-969.

Research output: Contribution to journalArticle

Iwamoto, Tsutomu ; Yamada, Aya ; Yuasa, Kenji ; Fukumoto, Emiko ; Nakamura, Takashi ; Fujiwara, Taku ; Fukumoto, Satoshi. / Influences of interferon-gamma on cell proliferation and interleukin-6 production in Down syndrome derived fibroblasts. In: Archives of Oral Biology. 2009 ; Vol. 54, No. 10. pp. 963-969.
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