Inhibition by haloperidol, of adenosine 5'-triphosphate-evoked responses in rat pheochromocytoma cells

Schuichi Koizumi, Makoto Ikeda, Ken Nakazawa, Kaori Inoue, Kanako Ito, Kazuhide Inoue

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effects of haloperidol, a typical antipsychotic drug, on adenosine 5'-triphosphate (ATP)-induced increase in intracellular Ca2+ concentration ([Ca]i) and inward currents were investigated using rat pheochromocytoma PC12 cells. Haloperidol (10-100 μM) significantly attenuated the rise in [Ca]i evoked by 30 μM ATP. This inhibition was not mimicked by (-)-sulpiride, an antagonist to dopamine D2-receptor. Dopamine or other dopaminergic agents did not affect the [Ca]i rise evoked by ATP. Under whole-cell voltage-clamp, haloperidol inhibited the ATP-activated current in the cells. These results suggest that haloperidol inhibits the ATP-receptor/channel, leading to the attenuation of the ATP-evoked increase in [Ca]i. Mechanisms involving dopamine receptors may not contribute to the inhibition by haloperidol.

Original languageEnglish
Pages (from-to)624-630
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume210
Issue number2
DOIs
Publication statusPublished - Jan 1 1995

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Pheochromocytoma
Haloperidol
Adenosine
Rats
Adenosine Triphosphate
Sulpiride
Dopamine Agents
Dopamine D2 Receptors
PC12 Cells
Dopamine Receptors
Clamping devices
Antipsychotic Agents
triphosphoric acid
Dopamine
Electric potential

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Inhibition by haloperidol, of adenosine 5'-triphosphate-evoked responses in rat pheochromocytoma cells. / Koizumi, Schuichi; Ikeda, Makoto; Nakazawa, Ken; Inoue, Kaori; Ito, Kanako; Inoue, Kazuhide.

In: Biochemical and Biophysical Research Communications, Vol. 210, No. 2, 01.01.1995, p. 624-630.

Research output: Contribution to journalArticle

Koizumi, Schuichi ; Ikeda, Makoto ; Nakazawa, Ken ; Inoue, Kaori ; Ito, Kanako ; Inoue, Kazuhide. / Inhibition by haloperidol, of adenosine 5'-triphosphate-evoked responses in rat pheochromocytoma cells. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 210, No. 2. pp. 624-630.
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