Inhibition by imipramine of ATP-evoked responses in rat pheochromocytoma cells

Schuichi Koizumi; Hisayuki Uneyama; Makoto Ikeda; Sinya Ueno; Kazuhide Inoue

doi:10.1006/bbrc.1998.8269

Inhibition by imipramine of ATP-evoked responses in rat pheochromocytoma cells

Schuichi Koizumi, Hisayuki Uneyama, Makoto Ikeda, Sinya Ueno, Kazuhide Inoue

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The effect of imipramine on the ATP-evoked release of dopamine was analyzed in parallel with its effects on the rise in the intracellular Ca²⁺ concentration ([Ca²⁺](i)) and current induced by ATP in rat pheochromocytoma PC12 cells. Imipramine (10-300 μM) inhibited the ATP-evoked release of dopamine and rise in [Ca²⁺l(i) in a concentration-dependent fashion though the effect of imipramine on the release was slightly more obvious. Imipramine also inhibited the ATP-activated inward current at a similar concentration range. These results show a new pharmacological profile of imipramine, namely the inhibition of P2X₂ receptors.

Original language	English
Pages (from-to)	342-346
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	244
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1998.8269
Publication status	Published - Mar 17 1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.1998.8269

Cite this

@article{f176ba5537164426a8791ce54acbdb6d,

title = "Inhibition by imipramine of ATP-evoked responses in rat pheochromocytoma cells",

abstract = "The effect of imipramine on the ATP-evoked release of dopamine was analyzed in parallel with its effects on the rise in the intracellular Ca2+ concentration ([Ca2+](i)) and current induced by ATP in rat pheochromocytoma PC12 cells. Imipramine (10-300 μM) inhibited the ATP-evoked release of dopamine and rise in [Ca2+l(i) in a concentration-dependent fashion though the effect of imipramine on the release was slightly more obvious. Imipramine also inhibited the ATP-activated inward current at a similar concentration range. These results show a new pharmacological profile of imipramine, namely the inhibition of P2X2 receptors.",

author = "Schuichi Koizumi and Hisayuki Uneyama and Makoto Ikeda and Sinya Ueno and Kazuhide Inoue",

year = "1998",

month = mar,

day = "17",

doi = "10.1006/bbrc.1998.8269",

language = "English",

volume = "244",

pages = "342--346",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Inhibition by imipramine of ATP-evoked responses in rat pheochromocytoma cells

AU - Koizumi, Schuichi

AU - Uneyama, Hisayuki

AU - Ikeda, Makoto

AU - Ueno, Sinya

AU - Inoue, Kazuhide

PY - 1998/3/17

Y1 - 1998/3/17

N2 - The effect of imipramine on the ATP-evoked release of dopamine was analyzed in parallel with its effects on the rise in the intracellular Ca2+ concentration ([Ca2+](i)) and current induced by ATP in rat pheochromocytoma PC12 cells. Imipramine (10-300 μM) inhibited the ATP-evoked release of dopamine and rise in [Ca2+l(i) in a concentration-dependent fashion though the effect of imipramine on the release was slightly more obvious. Imipramine also inhibited the ATP-activated inward current at a similar concentration range. These results show a new pharmacological profile of imipramine, namely the inhibition of P2X2 receptors.

AB - The effect of imipramine on the ATP-evoked release of dopamine was analyzed in parallel with its effects on the rise in the intracellular Ca2+ concentration ([Ca2+](i)) and current induced by ATP in rat pheochromocytoma PC12 cells. Imipramine (10-300 μM) inhibited the ATP-evoked release of dopamine and rise in [Ca2+l(i) in a concentration-dependent fashion though the effect of imipramine on the release was slightly more obvious. Imipramine also inhibited the ATP-activated inward current at a similar concentration range. These results show a new pharmacological profile of imipramine, namely the inhibition of P2X2 receptors.

UR - http://www.scopus.com/inward/record.url?scp=0032539767&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032539767&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1998.8269

DO - 10.1006/bbrc.1998.8269

M3 - Article

C2 - 9514927

AN - SCOPUS:0032539767

SN - 0006-291X

VL - 244

SP - 342

EP - 346

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Inhibition by imipramine of ATP-evoked responses in rat pheochromocytoma cells

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this