Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1e-115)

Futoshi Shintani, Shigenobu Kanba, Toshio Nakaki, Ryoko Nakamura, Koichi Sato, Gohei Yagi, Elliott Richelson, Ryuichi Kato, Masahiro Asai

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Abstract

We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, NIE-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [3H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 11M L-NG-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor- activated cyclic GMP formation in the cells. In addition, Li+ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalNeuropsychopharmacology
Volume11
Issue number2
DOIs
Publication statusPublished - Oct 1994

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Psychiatry and Mental health

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    Shintani, F., Kanba, S., Nakaki, T., Nakamura, R., Sato, K., Yagi, G., Richelson, E., Kato, R., & Asai, M. (1994). Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1e-115). Neuropsychopharmacology, 11(2), 119-124. https://doi.org/10.1038/npp.1994.41