Inhibition of Alzheimer amyloid aggregation with sulfated gycopolymers

Yoshiko Miura, Kikuko Yasuda, Kiyofumi Yamamoto, Mihoko Koike, Yoshihiro Nishida, Kazukiyo Kobayashi

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Glycopolymers carrying sulfated saccharides with modest sugar contents (11% and 28%) were found to suppress the formation of amyloid fibrils by amyloid β peptides (Aβ(1-42), Aβ(1-40), and Aβ(25-35)), as evaluated by thioflavin T assays and atomic force microscopy observation. Circular dichroism spectra showed that the conformation of amyloid β peptides depended on the glycopolymer additives, and that the glycopolymer additives reduced the β-sheet contents. Neutralization activity was confirmed by in vitro assay with HeLa cells. The sulfate group and the appropriate sugar contents were essential for the inhibitory effect.

Original languageEnglish
Pages (from-to)2129-2134
Number of pages6
JournalBiomacromolecules
Volume8
Issue number7
DOIs
Publication statusPublished - Jul 1 2007
Externally publishedYes

Fingerprint

Amyloid
Agglomeration
Sugars
Peptides
Assays
Atomic Force Microscopy
Dichroism
Circular Dichroism
HeLa Cells
Sulfates
Conformations
Atomic force microscopy
Observation

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Miura, Y., Yasuda, K., Yamamoto, K., Koike, M., Nishida, Y., & Kobayashi, K. (2007). Inhibition of Alzheimer amyloid aggregation with sulfated gycopolymers. Biomacromolecules, 8(7), 2129-2134. https://doi.org/10.1021/bm0701402

Inhibition of Alzheimer amyloid aggregation with sulfated gycopolymers. / Miura, Yoshiko; Yasuda, Kikuko; Yamamoto, Kiyofumi; Koike, Mihoko; Nishida, Yoshihiro; Kobayashi, Kazukiyo.

In: Biomacromolecules, Vol. 8, No. 7, 01.07.2007, p. 2129-2134.

Research output: Contribution to journalArticle

Miura, Y, Yasuda, K, Yamamoto, K, Koike, M, Nishida, Y & Kobayashi, K 2007, 'Inhibition of Alzheimer amyloid aggregation with sulfated gycopolymers', Biomacromolecules, vol. 8, no. 7, pp. 2129-2134. https://doi.org/10.1021/bm0701402
Miura Y, Yasuda K, Yamamoto K, Koike M, Nishida Y, Kobayashi K. Inhibition of Alzheimer amyloid aggregation with sulfated gycopolymers. Biomacromolecules. 2007 Jul 1;8(7):2129-2134. https://doi.org/10.1021/bm0701402
Miura, Yoshiko ; Yasuda, Kikuko ; Yamamoto, Kiyofumi ; Koike, Mihoko ; Nishida, Yoshihiro ; Kobayashi, Kazukiyo. / Inhibition of Alzheimer amyloid aggregation with sulfated gycopolymers. In: Biomacromolecules. 2007 ; Vol. 8, No. 7. pp. 2129-2134.
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