Inhibition of intercellular communication via gap junction in cultured aortic endothelial cells by elevated glucose and phorbol ester

Toyoshi Inoguchi, Fusao Ueda, Fumio Umeda, Tsukasa Yamashita, Hajime Nawata

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    48 Citations (Scopus)

    Abstract

    Gap junctional intercellular communication (GJIC) is important in coordinating the cells in maintaining tissue homeostasis and in regulating signal transmission. We examined the effect of elevated glucose on GJIC activity in cultured bovine aortic endothelial cells. GJIC activity was assessed by quantitating the transfer from cell to cell of directly microinjected fluorescent dye molecules. GJIC was activated in the subconfluent monolayer. In this condition, exposing the cells to elevated glucose (400 mg/dl) for 24 hrs significantly inhibited GJIC activity, as compared with low glucose (100 mg/dl). This inhibition of GJIC activity induced by elevated glucose was mimicked by addition of 12-O-tetradecanoylphorbol-13-acetate and was restored by addition of staurosporin (10-8 M), a PKC inhibitor. These results suggest that inhibition of GJIC activity induced by elevated glucose probably through activation of PKC may be involved in the vascular endothelial cell dysfunction associated with diabetes.

    Original languageEnglish
    Pages (from-to)492-497
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume208
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 1995

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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