Inhibition of Ion Channels by Hirsutine in Rat Pheochromocytoma Cells

Ken Nakazawa, Tomokazu Watano, Mica Ohara-Imaizumi, Kazuhide Inoue, Kannosuke Fujimori, Yukihiro Ozaki, Masatoshi Harada, Akira Takanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Effects of hirsutine, an alkaloid that produces a potent ganglion blocking effcct, were investigated using rat pheochromocytoma PC12 cells. Hirsutine (1 to 10μM) suppressed dopamine-release evoked by 100 μM nicotine. In voltage-clamped cells, hirsutine (1 to 10 μM) inhibited the inward current activated by 100 μM nicotine. Hirsutine’ was equipotent to hexamethonium in blocking the nicotine-activated current. The voltage-dependency of the nicotine activated current was not modified by hirsutine. Effects of hirustine on other ion channels were tested to determire its selectivity. Inward currents mediated through ATP-activated channels were scarcely affected by hirsutine (up to 100 μM). However, hirustine (10μM) inhibited Ba currents passing through Ca channels and K currents activated by depolarizing voltage steps. The results suggest that hirsutine potently blocks nicotinic receptor-channels, but hirsutine also inhibits voltage-gated Ca and K channels. Roles of the inhibition of these channels in the pharmacological effects of hirsutine were discussed.

Original languageEnglish
Pages (from-to)507-515
Number of pages9
Journalthe japanese journal of pharmacology
Volume57
Issue number4
DOIs
Publication statusPublished - Jan 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology

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    Nakazawa, K., Watano, T., Ohara-Imaizumi, M., Inoue, K., Fujimori, K., Ozaki, Y., Harada, M., & Takanaka, A. (1991). Inhibition of Ion Channels by Hirsutine in Rat Pheochromocytoma Cells. the japanese journal of pharmacology, 57(4), 507-515. https://doi.org/10.1254/jjp.57.507