Inhibition of leukotriene formation in human leukocytes by halothane

Yamaoka Atsushi, Hideki Sumimoto, Takeshige Koichiro, Itoh Takeo, Isobe Ryuichi, Yoshitake Junichi, Minakami Shigeki

Research output: Contribution to journalArticle

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Abstract

The effects of an inhalation anesthetic, halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) on the formation of 5-lipoxygenase metabolites such as leukotriene B4, 5(S)-hydroxyeicosatetraenoic acid (5-HETE), 6-trans-isomers of leukotriene B4 and leukotriene C4 were studied in human leukocytes stimulated with calcium ionophore A23187. Halothane inhibited the formation of all these metabolites dose dependently and the formation was restored by removal of the drug. The anesthetic also reversibly inhibited the release of [3H]arachidonic acid from neutrophils with a half-inhibition concentration of less than 0.19 mM. The formation of 5-lipoxygenase metabolites was not inhibited by the anesthetic when leukocytes were stimulated with the ionophore in the presence of exogenous arachidonic acid. These observations indicate that the inhibitory effect of halothane on the formation of 5-lipoxygenase metabolites in leukocytes is mainly due to the inhibition of arachidonic acid release.

Original languageEnglish
Pages (from-to)284-292
Number of pages9
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume918
Issue number3
DOIs
Publication statusPublished - Apr 24 1987

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Arachidonate 5-Lipoxygenase
Leukotrienes
Halothane
Metabolites
Arachidonic Acid
Leukocytes
Leukotriene B4
Anesthetics
Inhalation Anesthetics
Leukotriene C4
Calcium Ionophores
Ionophores
Calcimycin
Hydroxyeicosatetraenoic Acids
Neutrophils
Isomers
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

Inhibition of leukotriene formation in human leukocytes by halothane. / Atsushi, Yamaoka; Sumimoto, Hideki; Koichiro, Takeshige; Takeo, Itoh; Ryuichi, Isobe; Junichi, Yoshitake; Shigeki, Minakami.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 918, No. 3, 24.04.1987, p. 284-292.

Research output: Contribution to journalArticle

Atsushi, Yamaoka ; Sumimoto, Hideki ; Koichiro, Takeshige ; Takeo, Itoh ; Ryuichi, Isobe ; Junichi, Yoshitake ; Shigeki, Minakami. / Inhibition of leukotriene formation in human leukocytes by halothane. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1987 ; Vol. 918, No. 3. pp. 284-292.
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