Inhibition of mitogen activated protein kinase activity induces parthenogenetic activation and increases cyclin B accumulation during porcine oocyte maturation

Ikuko Takakura, Kunihiko Naito, Naoki Iwamori, Masakane Yamashita, Sachi Kume, Hideaki Tojo

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12 Citations (Scopus)

Abstract

The inhibition of mitogen activated protein kinase (MAPK) activation during porcine oocyte maturation leads to decreased maturation promoting factor (MPF) activity and to the induction of parthenogenetic activation. In the present study, in order to analyze the mechanism underlying the suppression of MPF activity in MAPK-inhibited porcine oocytes, we injected mRNA of SASA-MEK, a dominant negative MAPK kinase, or antisense RNA of c-mos, a MAPK kinase kinase, into immature porcine oocyte cytoplasm. The injection of SASA-MEK mRNA or c-mos antisense RNA inhibited the MAPK activity partially or completely, respectively, decreased the MPF activity slightly or significantly, respectively, and induced parthenogenetic activation in 17.1% or 96.6% of mature oocytes, respectively, although no parthenogenetic activation was observed in the control oocytes. Immunoblotting experiments revealed that cyclin B accumulation in these MAPK-suppressed porcine oocytes was increased significantly after 50 h of culture and that a considerable amount of MPF was converted into inactive pre-MPF by hyperphosphorylation. These results indicate that the inhibition of MAPK activity in porcine oocytes did not promote cyclin B degradation but rather suppressed it; also the decrease in MPF activity in MAPK-suppressed porcine oocytes correlated with the conversion of active MPF into inactive pre-MPF.

Original languageEnglish
Pages (from-to)617-626
Number of pages10
JournalJournal of Reproduction and Development
Volume51
Issue number5
DOIs
Publication statusPublished - Oct 1 2005

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maturation-promoting factor
cyclins
mitogen-activated protein kinase
oocytes
swine
antisense RNA
mitogen-activated protein kinase kinase kinase
mitogen-activated protein kinase kinase
immunoblotting
cytoplasm
immatures
injection

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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Inhibition of mitogen activated protein kinase activity induces parthenogenetic activation and increases cyclin B accumulation during porcine oocyte maturation. / Takakura, Ikuko; Naito, Kunihiko; Iwamori, Naoki; Yamashita, Masakane; Kume, Sachi; Tojo, Hideaki.

In: Journal of Reproduction and Development, Vol. 51, No. 5, 01.10.2005, p. 617-626.

Research output: Contribution to journalArticle

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AU - Tojo, Hideaki

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