Inhibition of neuraminidase by Ganoderma triterpenoids and implications for neuraminidase inhibitor design

Qinchang Zhu, Tran Hai Bang, Koichiro Ohnuki, Takashi Sawai, Ken Sawai, Kuniyoshi Shimizu

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Neuraminidase (NA) inhibitors are the dominant antiviral drugs for treating influenza in the clinic. Increasing prevalence of drug resistance makes the discovery of new NA inhibitors a high priority. Thirty-one triterpenoids from the medicinal mushroom Ganoderma lingzhi were analyzed in an in vitro NA inhibition assay, leading to the discovery of ganoderic acid T-Q and TR as two inhibitors of H5N1 and H1N1 NAs. Structure-activity relationship studies revealed that the corresponding triterpenoid structure is a potential scaffold for the design of NA inhibitors. Using these triterpenoids as probes we found, through further in silico docking and interaction analysis, that interactions with the amino-acid residues Arg292 and/or Glu119 of NA are critical for the inhibition of H5N1 and H1N1. These findings should prove valuable for the design and development of NA inhibitors.

Original languageEnglish
Article number13194
JournalScientific reports
Volume5
DOIs
Publication statusPublished - Aug 26 2015

All Science Journal Classification (ASJC) codes

  • General

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