Inhibition of Pancreatic Hepatocyte Induction in Hamsters Treated Sequentially with Ethionine, a Protein‐free Diet and then Methionine by Prior N‐nitrosobis‐(2‐oxopropyI)amine Administration

Shunji Kitazawa, Kazuhiro Mizumoto, Toshihiro Amanuma, Masahiro Tsutsumi, Hiroshi Maruyama, Yoichi Konishi

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Abstract

A 100% yield of pancreatic hepatocytes was induced in pancreas tissues of female hamsters treated with twice‐repeated sequential administrations of DL‐ethionine (ethionine) together with a protein‐free diet and then L‐methionine (methionine) for 10 weeks. The cells were also found in 40% of hamsters receiving 20mg/kg body weight of the pancreatic carcinogen, N nitrosobis(2‐oxopropyl)amine (BOP) given twice at the peak of pancreatic regeneration stimulated by methionine after ethionine induced cell damage. However, BOP at doses of 30, 70, and 100 mg/kg body weight administered before the occurrence of pancreatic regeneration dose dependently inhibited their appearance, with reduction of the yield to 40%, 25%, and 8.3% respectively, and BOP per se did not induce any development of pancreatic hepatocytes. Stein iodine staining revealed bile pigments in the induced hamster pancreatic eosinophilic cell populations.

Original languageEnglish
Pages (from-to)562-567
Number of pages6
JournalPathology International
Volume40
Issue number8
DOIs
Publication statusPublished - Aug 1990

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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