The effect of 4,5-dioxovaleric acid on the activity of porphobilinogen (PBG) synthase (formerly 5-aminolevulinic acid dehydratase, EC 18.104.22.168) of the porphyrin synthetic pathway was studied with the enzyme purified from Chlorella regularis. 4,5-Dioxovaleric acid, a metabolite of 5-aminolevulinic acid, competitively inhibited PBG synthase with a Ki value of 1.4 mm. The concentration for the half inhibition of 4,5-dioxovaleric acid (7 mm) was slightly lower than that for the known competitive inhibitor, levulinic acid (12 mm).
|Number of pages||4|
|Journal||Plant and Cell Physiology|
|Publication status||Published - Mar 1985|
All Science Journal Classification (ASJC) codes
- Plant Science
- Cell Biology