Inhibition of porphobilinogen synthase activity by 4,5-dioxovalerate

Yuzo Shioi, Michio Doi, Tsutomu Sasa

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Abstract

The effect of 4,5-dioxovaleric acid on the activity of porphobilinogen (PBG) synthase (formerly 5-aminolevulinic acid dehydratase, EC 4.2.1.24) of the porphyrin synthetic pathway was studied with the enzyme purified from Chlorella regularis. 4,5-Dioxovaleric acid, a metabolite of 5-aminolevulinic acid, competitively inhibited PBG synthase with a Ki value of 1.4 mm. The concentration for the half inhibition of 4,5-dioxovaleric acid (7 mm) was slightly lower than that for the known competitive inhibitor, levulinic acid (12 mm).

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalPlant and Cell Physiology
Volume26
Issue number2
Publication statusPublished - Mar 1 1985

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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