Inhibition of porphyrin biosynthesis by exogenous 5-aminolevulinic acid in an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114

Yuzo Shioi, Michio Doi, Kimiko Tanabe, Keishi Shimokawa

Research output: Contribution to journalArticle

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Abstract

Exogenously administrated 5-aminolevulinic acid (ALA) inhibited the formation of bacteriochlorophyll a (Bchl a) in a dose-dependent manner in the aerobic photosynthetic bacterium, Erythrobacter sp. strain OCh 114, under dark growth conditions. The ALA concentration required for half-inhibition after 24-h growth was estimated to be about 3.0 mm. Porphyrin and Bchl precursors were not found in either the cells or the growth medium. The same inhibition was also observed with cytochrome c formation. When ALA was incubated with intact cells, a large amount of ALA was converted to an unknown metabolite. The pH optimum of the conversion was 7.8. The metabolite did not react with Ehrlich's reagent, but did so with ninhydrin, giving a yellow color. Based on analyses by several techniques including mass spectrometry, ir spectrometry, and paper electrophoresis, it was identified as 4-hydroxy-5-aminovaleric acid (HAVA). Authentic HAVA prepared from ALA was a competitive inhibitor of the enzyme, porphobilinogen synthase of Erythrobacter. The Ki value for authentic HAVA was calculated to be 2.4 mm from a Dixon plot and the HAVA concentration required for half-inhibition was 17 mm. It is concluded that in Erythrobacter cells, exogenous ALA is converted to the metabolite, HAVA, which is responsible for the inhibition of porphobilinogen synthase as well as that of Bchl a and cytochrome formation.

Original languageEnglish
Pages (from-to)478-485
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume266
Issue number2
DOIs
Publication statusPublished - Nov 1 1988

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Sphingomonadaceae
Aerobic Bacteria
Aminolevulinic Acid
Biosynthesis
Porphyrins
Bacteria
Metabolites
Porphobilinogen Synthase
Bacteriochlorophylls
Growth
Ninhydrin
Paper Electrophoresis
Enzyme Inhibitors
Cytochromes
Cytochromes c
Electrophoresis
Spectrometry
Mass spectrometry
Mass Spectrometry
Spectrum Analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

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Inhibition of porphyrin biosynthesis by exogenous 5-aminolevulinic acid in an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114. / Shioi, Yuzo; Doi, Michio; Tanabe, Kimiko; Shimokawa, Keishi.

In: Archives of Biochemistry and Biophysics, Vol. 266, No. 2, 01.11.1988, p. 478-485.

Research output: Contribution to journalArticle

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