Inhibition of Pseudorabies Virus Replication by a Chimeric trans-gene Product Repressing Transcription of the Immediate-Early Gene

Etsuro Ono, Yoshihiro Sakoda, Satoshi Taharaguchi, Shinji Watanabe, Noriko Tonomura, Hiroshi Kida, Yukio Shimizu

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A chimeric gene encoding a fusion protein consisting of the DNA-binding domain of the immediate-early (IE) protein of pseudorabies virus (PRV) and a tail-truncated Vmw65 of herpes simplex virus 1, lacking the transcription activation domain, was constructed The chimeric gene product inhibited transcription from the PRV IE promoter in a transient expression assay. A HeLa cell line stably transformed with the chimeric gene showed remarkable resistance to PRV infection In the transformed cells infected with PRV, transcription of the PRV IE gene was repressed, indicating that the resistance of the cells to PRV infection was due to interference with IE gene transcription by the fusion protein.

Original languageEnglish
Article number71324
Pages (from-to)128-140
Number of pages13
JournalVirology
Volume210
Issue number1
DOIs
Publication statusPublished - Jun 20 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

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