@article{7f8e4b2e3aff4d8a962d39ab4a864a6c,
title = "Inhibition of PTEN tumor suppressor promotes the generation of induced pluripotent stem cells",
abstract = "Induced pluripotent stem cells (iPSCs) can be generated from patients with specific diseases by the transduction of reprogramming factors and can be useful as a cell source for cell transplantation therapy for various diseases with impaired organs. However, the low efficiency of iPSC derived from somatic cells (0.01-0.1%) is one of the major problems in the field. The phosphoinositide 3-kinase (PI3K) pathway is thought to be important for self-renewal, proliferation, and maintenance of embryonic stem cells (ESCs), but the contribution of this pathway or its well-known negative regulator, phosphatase, and tensin homolog deleted on chromosome ten (Pten), to somatic cell reprogramming remains largely unknown. Here, we show that activation of the PI3K pathway by the Pten inhibitor, dipotassium bisperoxo(5-hydroxypyridine-2- carboxyl)oxovanadate, improves the efficiency of germline-competent iPSC derivation from mouse somatic cells. This simple method provides a new approach for efficient generation of iPSCs.",
author = "Jiyuan Liao and Tomotoshi Marumoto and Saori Yamaguchi and Shinji Okano and Naoki Takeda and Chika Sakamoto and Hirotaka Kawano and Takenobu Nii and Shohei Miyamato and Yoko Nagai and Michiyo Okada and Hiroyuki Inoue and Kohichi Kawahara and Akira Suzuki and Yoshie Miura and Kenzaburo Tani",
note = "Funding Information: The authors thank Ken-ichi Yamamura (Scientific Support Programs for Cancer Research Grant-in-Aid for Scientific Research on Innovative Areas, Kumamoto University, Japan), and Masato Tanaka and Kaori Nagatoshi for their help to establish chimeras. They also thank Michiko Ushijima for administrative assistance, Atsushi Takahashi and the members of Kenzaburo Tani's laboratory for providing constructive criticism and technical assistance, and Peng Xiong (Kyushu University) for his help in performing statistical analysis. They also thank Hiroyuki Sasaki (Kyushu University) for helpful discussions and providing bisulfite PCR primers for Nanog. This work was partly performed in the Cooperative Research Project Program of the Medical Institute of Bioregulation, Kyushu University, the Research Support Center (Graduate School of Medical Sciences, Kyushu University) and Laboratory for Technical Support (Medical Institute of Bioregulation, Kyushu University) for technical assistance. This work was supported by grants from the Project for Realization of Regenerative Medicine (K.T., 08008010) and KAKENHI (T.M., 23590465) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan. The authors declare no conflict of interest.",
year = "2013",
month = jun,
doi = "10.1038/mt.2013.60",
language = "English",
volume = "21",
pages = "1242--1250",
journal = "Molecular Therapy",
issn = "1525-0016",
publisher = "Nature Publishing Group",
number = "6",
}