Inhibition of S-Phase entry of human fibroblasts by an antisense oligomer against hCDC47

Masatoshi Fujita, Tohru Kiyono, Yasuyuki Hayashi, Masahide Ishibashi

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17 Citations (Scopus)

Abstract

The MCM protein family has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Recently, we have identified human (h) CDC47, another member of the family, and demonstrated its unique S-phase nuclear association pattern implying participation in the regulation of DNA replication. In the present study, we applied an antisense strategy to assess the phenotype of human cells in which hCDC47 is repressed. As a result serum-induced accumulation of hCDC47 protein in human fibroblast cells was reduced by a specific antisense oligodeoxynucleotide and S-phase entry of such cells was significantly inhibited, indicating that hCDC47 protein is essential for DNA replication.

Original languageEnglish
Pages (from-to)604-607
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume219
Issue number2
DOIs
Publication statusPublished - Feb 15 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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