Inhibition of sprouty2 polarizes macrophages toward an M2 phenotype by stimulation with interferon γ and Porphyromonas gingivalis lipopolysaccharide

Ryo Atomura, Terukazu Sanui, Takao Fukuda, Urara Tanaka, Kyosuke Toyoda, Takaharu Taketomi, Kensuke Yamamichi, Hajime Akiyama, Fusanori Nishimura

Research output: Contribution to journalArticle

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Abstract

Periodontitis is a chronic inflammatory disorder caused by specific bacteria residing in the biofilm, particularly Porphyromonas gingivalis (Pg). Sprouty2 (Spry2) functions as a negative regulator of the fibroblast growth factor (FGF) signaling pathway. We previously demonstrated that sequestration of Spry2 induced proliferation and osteogenesis in osteoblastic cells by basic FGF (bFGF) and epidermal growth factor (EGF) stimulation in vitro, but diminished cell proliferation in gingival epithelial cells. In addition, Spry2 knockdown in combination with bFGF and EGF stimulation increases periodontal ligament cell proliferation and migration accompanied by prevention of osteoblastic differentiation. In this study, we investigated the mechanisms through which Spry2 depletion by interferon (IFN) γ and Pg lipopolysaccharide (LPS) stimulation affected the physiology of macrophages in vitro. Transfection of macrophages with Spry2 small-interfering RNA (siRNA) promoted the expression of genes characteristic of M2 alternative activated macrophages, induced interleukin (IL)-10 expression, and enhanced arginase activity, even in cells stimulated with IFNγ and Pg LPS. In addition, we found that phosphoinositide 3-kinase (PI3K) and AKT activation by Spry2 downregulation enhanced efferocytosis of apoptotic cells by increasing Rac1 activation and decreasing nuclear factor kappa B (NFkB) p65 phosphorylation but not signal transducer and activator of transcription 1 (STAT1) phosphorylation. Collectively, our results suggested that topical administration of Spry2 inhibitors may efficiently resolve inflammation in periodontal disease as macrophage-based anti-inflammatory immunotherapy and may create a suitable environment for periodontal wound healing. These in vitro findings provide a molecular basis for new therapeutic approaches in periodontal tissue regeneration.

Original languageEnglish
Pages (from-to)98-110
Number of pages13
JournalImmunity Inflammation and Disease
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Porphyromonas gingivalis
Interferons
Lipopolysaccharides
Macrophages
Phenotype
Epidermal Growth Factor
Phosphorylation
Cell Proliferation
STAT1 Transcription Factor
Topical Administration
Arginase
Periodontal Ligament
1-Phosphatidylinositol 4-Kinase
Fibroblast Growth Factors
NF-kappa B
Periodontitis
Periodontal Diseases
Fibroblast Growth Factor 2
Biofilms
Osteogenesis

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Inhibition of sprouty2 polarizes macrophages toward an M2 phenotype by stimulation with interferon γ and Porphyromonas gingivalis lipopolysaccharide. / Atomura, Ryo; Sanui, Terukazu; Fukuda, Takao; Tanaka, Urara; Toyoda, Kyosuke; Taketomi, Takaharu; Yamamichi, Kensuke; Akiyama, Hajime; Nishimura, Fusanori.

In: Immunity Inflammation and Disease, Vol. 4, No. 1, 01.01.2016, p. 98-110.

Research output: Contribution to journalArticle

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