Inhibition of TGF-β signaling exacerbates early cardiac dysfunction but prevents late remodeling after infarction

Masaki Ikeuchi, Hiroyuki Tsutsui, Tetsuya Shiomi, Hidenori Matsusaka, Shoji Matsushima, Jing Wen, Toru Kubota, Akira Takeshita

Research output: Contribution to journalArticle

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Abstract

Transforming growth factor (TGF)-β promotes the deposition of extracellular matrix protein and also acts as an anti-inflammatory cytokine. These biological effects might be involved in the development and progression of left ventricular (LV) remodeling and failure after myocardial infarction (MI). However, its pathophysiological significance remains obscure in post-MI hearts. Anterior MI was produced in mice by ligating the left coronary artery. TGF-β mRNA levels increased in both infarcted and noninfarcted LV after MI. To block TGF-β signaling during the early phase of MI, an extracellular domain of TGF-β type II receptor (TβIIR) plasmid was transfected into the limb skeletal muscles 7 days before ligation. TβIIR increased the mortality during 24 h of MI, as well as exacerbated LV dilatation and contractile dysfunction, the infiltration of neutrophils, and gene expression of tumor necrosis factor-α, interleukin-1β, and monocyte chemoattractant protein-1 compared with nontreated MI mice despite the comparable infarct size. Next, to block TGF-β signaling during the later phase, TβIIR was transfected into mice at days 0 and 7 after ligation. At 4 weeks, LV dilatation and contractile dysfunction in association with myocyte hypertrophy and interstitial fibrosis of noninfarcted LV seen in MI mice were prevented by TβIIR. The activation of TGF-β is protective against ischemic myocardial damage during the early phase. However, the beneficial effects might be lost, when its expression is sustained, thereby leading to LV remodeling and failure after MI.

Original languageEnglish
Pages (from-to)526-535
Number of pages10
JournalCardiovascular research
Volume64
Issue number3
DOIs
Publication statusPublished - Dec 1 2004

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Transforming Growth Factors
Infarction
Myocardial Infarction
Ventricular Remodeling
Ligation
Dilatation
Growth Factor Receptors
Neutrophil Infiltration
Chemokine CCL2
Extracellular Matrix Proteins
Interleukin-1
Muscle Cells
Hypertrophy
Coronary Vessels
Skeletal Muscle
Plasmids
Fibrosis
Anti-Inflammatory Agents
Extremities
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Inhibition of TGF-β signaling exacerbates early cardiac dysfunction but prevents late remodeling after infarction. / Ikeuchi, Masaki; Tsutsui, Hiroyuki; Shiomi, Tetsuya; Matsusaka, Hidenori; Matsushima, Shoji; Wen, Jing; Kubota, Toru; Takeshita, Akira.

In: Cardiovascular research, Vol. 64, No. 3, 01.12.2004, p. 526-535.

Research output: Contribution to journalArticle

Ikeuchi, Masaki ; Tsutsui, Hiroyuki ; Shiomi, Tetsuya ; Matsusaka, Hidenori ; Matsushima, Shoji ; Wen, Jing ; Kubota, Toru ; Takeshita, Akira. / Inhibition of TGF-β signaling exacerbates early cardiac dysfunction but prevents late remodeling after infarction. In: Cardiovascular research. 2004 ; Vol. 64, No. 3. pp. 526-535.
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