Inhibition of the Arabidopsis bHLH transcription factor by monomerization through abscisic acid-induced phosphorylation

Yohei Takahashi, Toshinori Kinoshita, Masaki Matsumoto, Ken Ichiro Shimazaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have demonstrated that the Arabidopsis basic helix-loop-helix (bHLH) transcription factor, ABA-responsive kinase substrate 1 (AKS1; also known as FLOWERING BHLH 3, FBH3), enhances K(+) channel expression in guard cells leading to stomatal opening. The expression is suppressed by ABA-induced phosphorylation of AKS1. Here we show that the phosphorylation results in the monomerization of AKS1 multimers and inhibits AKS1 binding to DNA. AKS1 forms homo-multimers which dissociate following phosphorylation. Replacement of a critical amino acid in the bHLH domain inhibited multimer formation and decreased the binding of AKS1 to DNA. The monomerization was elicited via phosphorylation at three serine residues, which is mediated by SNF1-related protein kinase 2.6 (SnRK2.6), in the vicinity of bHLH domain. Furthermore, ABA induced the phosphorylation-dependent release of AKS1 from DNA, thereby suppressing transcriptional activity in vivo. Our results document a mechanism that inhibits gene expression by phosphorylation of a bHLH transcription factor.

Original languageEnglish
Pages (from-to)559-567
Number of pages9
JournalThe Plant journal : for cell and molecular biology
Volume87
Issue number6
DOIs
Publication statusPublished - Sep 1 2016

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basic helix-loop-helix transcription factors
Basic Helix-Loop-Helix Transcription Factors
Abscisic Acid
Arabidopsis
abscisic acid
phosphorylation
Phosphorylation
Helix-Loop-Helix Motifs
DNA
Basic Amino Acids
potassium channels
guard cells
Homo
protein kinases
serine
Serine
phosphotransferases (kinases)
Phosphotransferases
Gene Expression
gene expression

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Inhibition of the Arabidopsis bHLH transcription factor by monomerization through abscisic acid-induced phosphorylation. / Takahashi, Yohei; Kinoshita, Toshinori; Matsumoto, Masaki; Shimazaki, Ken Ichiro.

In: The Plant journal : for cell and molecular biology, Vol. 87, No. 6, 01.09.2016, p. 559-567.

Research output: Contribution to journalArticle

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