Inhibition of the serotonin-induced inward current by dextromethorphan in rat nodose ganglion neurons

Hitoshi Ishibashi, Kei Eto, Yukiko Arimura, Jun Yamada, Yoshiko Hatano, Masazumi Nishikawa, Mami Noda, Kazuo Takahama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dextromethorphan is one of the most widely used antitussives for the treatment of cough. In the present study, we investigated the effect of dextromethorphan on 5-hydroxytryptamine (5-HT)-induced currents in acutely dissociated rat nodose ganglion neurons using nystatin-perforated patch-clamp recording configuration. The 5-HT-induced current was inhibited by the 5-HT3 receptor antagonist tropisetron, while the selective 5-HT3 receptor agonist 1-(m-chlorophenyl)-biguanide hydrochloride (mCPBG) induced a similar current. Dextromethorphan reversibly and concentration-dependently inhibited the 5-HT-induced inward current. The inhibition did not appear to be voltage-dependent. Both the peak and steady-state 5-HT-induced currents were inhibited by dextromethorphan, although the peak current was more sensitive to dextromethorphan block. The IC50 values for the inhibition of peak and steady currents evoked by 3 μM 5-HT were 16.4 and 34.4 μM, respectively. In the presence of 10 μM dextromethorphan, the concentration-response curve for 5-HT was shifted to the right without changing the maximum response, while high concentrations reduced the maximum current. The 5-HT EC50 values in the presence of 0, 10, 30 and 60 μM dextromethorphan were 4.3, 6.8, 15.5 and 40.6 μM, respectively. The results indicate that dextromethorphan inhibits the 5-HT-induced current of rat nodose ganglion neurons, and further suggest that dextromethorphan at a low concentration acts as a competitive inhibitor of 5-HT3 receptors.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalBrain Research
Volume1097
Issue number1
DOIs
Publication statusPublished - Jun 30 2006

Fingerprint

Nodose Ganglion
Dextromethorphan
Serotonin
Neurons
Receptors, Serotonin, 5-HT3
tropisetron
Serotonin 5-HT3 Receptor Agonists
Antitussive Agents
Serotonin 5-HT3 Receptor Antagonists
Nystatin
Cough
Inhibitory Concentration 50

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Inhibition of the serotonin-induced inward current by dextromethorphan in rat nodose ganglion neurons. / Ishibashi, Hitoshi; Eto, Kei; Arimura, Yukiko; Yamada, Jun; Hatano, Yoshiko; Nishikawa, Masazumi; Noda, Mami; Takahama, Kazuo.

In: Brain Research, Vol. 1097, No. 1, 30.06.2006, p. 65-70.

Research output: Contribution to journalArticle

Ishibashi, Hitoshi ; Eto, Kei ; Arimura, Yukiko ; Yamada, Jun ; Hatano, Yoshiko ; Nishikawa, Masazumi ; Noda, Mami ; Takahama, Kazuo. / Inhibition of the serotonin-induced inward current by dextromethorphan in rat nodose ganglion neurons. In: Brain Research. 2006 ; Vol. 1097, No. 1. pp. 65-70.
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