Inhibitory Effect of Leupeptin on the Intracellular Maturation of Lysosomal Cathepsin L in Primary Cultures of Rat Hepatocytes

Yukio Nishimura, Keitaro Kato, Masaru Himeno, Koji Furuno

Research output: Contribution to journalArticle

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Abstract

To investigate the intracellular processing event for lysosomal cathespin L, we examined the effect of leupeptin, a non-covalent cysteine proteinase inhibitor, on the intracellular processing kinetics of cathepsin L as analyzed by pulse-chase experiments in vivo with [35S] methionine in primary cultures of rat hepatocytes. This revealed that cathepsin L was initially synthesized as a proenzyme of molecular weight 39 kDa and the proenzyme was subsequently processed to the mature form of the enzyme, 30 and 25 kDa. In the leupeptin-treated cells, the proteolytic conversion of cellular procathepsin L, of molecular weight 39 kDa, to the mature enzyme was significantly inhibited and considerable amounts of proenzyme were found in the cell after 8 h chase periods. Furthermore, the subcellular fractionation experiment demonstrated that the intracellular processing of procathepsin L in the high density lysosomal fraction was significantly inhibited and that considerable amounts of the procathepsin L form were still observed in the dense lysosomal fraction after a 2 h chase period. These results suggest that leupeptin treatment caused significant inhibition of the intracellular maturation of cathepsin L. These findings show that cysteine proteinase plays an important role in the intracellular proteolytic processing and activation of lysosomal cathepsin L in vivo and that this processing event occurs within the lysosomes.

Original languageEnglish
Pages (from-to)945-950
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume18
Issue number7
DOIs
Publication statusPublished - Jan 1 1995

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Cathepsin L
Enzyme Precursors
Hepatocytes
Molecular Weight
Cysteine Proteinase Inhibitors
L Forms
Cysteine Proteases
Enzymes
Lysosomes
Methionine
leupeptin
procathepsin L

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Inhibitory Effect of Leupeptin on the Intracellular Maturation of Lysosomal Cathepsin L in Primary Cultures of Rat Hepatocytes. / Nishimura, Yukio; Kato, Keitaro; Himeno, Masaru; Furuno, Koji.

In: Biological and Pharmaceutical Bulletin, Vol. 18, No. 7, 01.01.1995, p. 945-950.

Research output: Contribution to journalArticle

Nishimura, Yukio ; Kato, Keitaro ; Himeno, Masaru ; Furuno, Koji. / Inhibitory Effect of Leupeptin on the Intracellular Maturation of Lysosomal Cathepsin L in Primary Cultures of Rat Hepatocytes. In: Biological and Pharmaceutical Bulletin. 1995 ; Vol. 18, No. 7. pp. 945-950.
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