Inhibitory effect of the tea polyphenol, (-)-epigallocatechin gallate, on growth of cervical adenocarcinoma cell lines

Mitsuyo Noguchi, Masatoshi Yokoyama, Sumiko Watanabe, Michiko Uchiyama, Yoshifumi Nakao, Kouichi Hara, Tsuyoshi Iwasaka

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

To investigate the effect of (-)-epigallocatechin gallate (EGCG) on cervical adenocarcinoma, we performed a cell proliferation assay. TRAP assay is used for telomerase activity, flow cytometry analysis and pKi-67 immunofluoroscein staining in cervical adenocarcinoma cell lines (OMC-4, TMCC-1). Our results showed that EGCG inhibited the proliferation assay, TRAP assay for telomerase activity of both cell lines. Although cell apoptosis was induced, we observed that the expression of pKi-67 was suppressed. Our data suggest that EGCG may be effective for the treatment of cervical adenocarcinoma. The mechanisms of the anti-tumor effects were revealed to be the inhibition of telomerase activity, the induction of apoptosis and cell cycle dysregulation.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalCancer Letters
Volume234
Issue number2
DOIs
Publication statusPublished - Mar 28 2006
Externally publishedYes

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Telomerase
Polyphenols
Tea
Adenocarcinoma
Cell Line
Growth
Apoptosis
Cell Cycle
Flow Cytometry
Cell Proliferation
Staining and Labeling
epigallocatechin gallate
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Inhibitory effect of the tea polyphenol, (-)-epigallocatechin gallate, on growth of cervical adenocarcinoma cell lines. / Noguchi, Mitsuyo; Yokoyama, Masatoshi; Watanabe, Sumiko; Uchiyama, Michiko; Nakao, Yoshifumi; Hara, Kouichi; Iwasaka, Tsuyoshi.

In: Cancer Letters, Vol. 234, No. 2, 28.03.2006, p. 135-142.

Research output: Contribution to journalArticle

Noguchi, Mitsuyo ; Yokoyama, Masatoshi ; Watanabe, Sumiko ; Uchiyama, Michiko ; Nakao, Yoshifumi ; Hara, Kouichi ; Iwasaka, Tsuyoshi. / Inhibitory effect of the tea polyphenol, (-)-epigallocatechin gallate, on growth of cervical adenocarcinoma cell lines. In: Cancer Letters. 2006 ; Vol. 234, No. 2. pp. 135-142.
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AU - Watanabe, Sumiko

AU - Uchiyama, Michiko

AU - Nakao, Yoshifumi

AU - Hara, Kouichi

AU - Iwasaka, Tsuyoshi

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