Inhibitory Effect on Thrombin-induced Myosin Light Chain Phosphorylation by Extract of a New Green Tea Cultivar in A7r5 Rat Aortic Smooth Muscle Cells

Kana Kurihara, Shunsuke Nagatomo, Mari Maeda-Yamamoto, Koji Yamada, Hirofumi Tachibana

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We show that an extract of a new green tea cultivar Cha Chuukanbohon Nou6 (Nou6), which rich in anthocyanin, has an inhibitory effect on myosin regulatory light chain (MRLC) phosphorylation in rat aortic smooth muscle cell line A7r5. The inhibitory effect of Nou6 extract was abolished when the extract was treated with polyvinylpyrrolidone which traps polyphenols. However, none of the major tea constituents tested had an inhibitory effect. In addition, we found that Nou6 extract had a strong effect on reducing the phosphorylation of myosin phosphatase targeting subunit 1 at Thr696. These results suggest that Nou6 extract inhibits MRLC phosphorylation induced by thrombin through activating myosin light chain phosphatase, and polyphenols in the tea extract may contribute to this effect.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalFood Science and Technology Research
Volume16
Issue number3
DOIs
Publication statusPublished - May 2010

Fingerprint

myosin light chains
Myosin Light Chains
Phosphorylation
thrombin
green tea
Tea
Myosin-Light-Chain Phosphatase
Thrombin
smooth muscle
myocytes
Smooth Muscle Myocytes
Muscle
Rats
phosphorylation
Phosphatases
Cells
Polyphenols
rats
extracts
cultivars

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

Cite this

Inhibitory Effect on Thrombin-induced Myosin Light Chain Phosphorylation by Extract of a New Green Tea Cultivar in A7r5 Rat Aortic Smooth Muscle Cells. / Kurihara, Kana; Nagatomo, Shunsuke; Maeda-Yamamoto, Mari; Yamada, Koji; Tachibana, Hirofumi.

In: Food Science and Technology Research, Vol. 16, No. 3, 05.2010, p. 263-266.

Research output: Contribution to journalArticle

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