Inhibitory effects of draxin on axonal outgrowth and migration of precerebellar neurons

M. Asrafuzzaman Riyadh, Yohei Shinmyo, Kunimasa Ohta, Hideaki Tanaka

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The rhombic lip, a dorsal stripe of the neuroepithelium lining the edge of the fourth ventricle, is the site of origin of precerebellar neurons (PCN), which migrate tangentially towards the floor plate. After reaching the floor plate, they project their axons to the cerebellum. Although previous studies have shown that the guidance molecules Netrin/DCC and Slit/Robo have critical roles in PCN migration, the molecular mechanisms underlying this process remain poorly understood. Here, we report that draxin, a repulsive axon guidance protein, is involved in PCN development. We found that draxin is expressed in the rhombic lip and migratory stream of some PCN in the developing hindbrain of mice. In addition, draxin inhibited neurite outgrowth and nuclei migration from rhombic lip explants. These results suggest that draxin functions as a repulsive guidance cue for PCN migration. However, we observed no significant differences in PCN distribution between draxin-/- and wild type embryos. Thus, draxin and other axon guidance cues may have redundant roles in PCN migration.

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume449
Issue number1
DOIs
Publication statusPublished - Jun 20 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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