Thirty stable hybridoma cell lines secreting monoclonal antibodies specific for Japanese radish acid phosphatase (EC 18.104.22.168) were obtained. These antibodies were inhibitory or stimulatory or had no effect on the enzyme activity. Four antibodies (IgG1 subclass) among them, designated MAb-11, MAb-18, MAb-20, and MAb-30, were purified and partially characterized. MAb-11, MAb-18, and MAb-20 inhibited more than 80% of the enzyme activity and appeared to act as noncompetitive inhibitors. Competitive inhibition assay indicated that MAb-18 and MAb-20 were classified into the same group and MAb-11 into another group. MAb-20 had an inhibitory effect on acid phosphatases from some vegetable and meat sources. The Fab fragment prepared by limited proteolysis of MAb-20 with papain also inhibited the enzyme.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry