Inhibitory Monoclonal Antibody against Japanese Radish Acid Phosphatase

Ryoji Takata, Makoto Yoshimoto, Takahisa Miyamoto, Shoji Hatano, Jiro Sakamoto, Takahiko Soeda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Thirty stable hybridoma cell lines secreting monoclonal antibodies specific for Japanese radish acid phosphatase (EC 3.1.3.2) were obtained. These antibodies were inhibitory or stimulatory or had no effect on the enzyme activity. Four antibodies (IgG1 subclass) among them, designated MAb-11, MAb-18, MAb-20, and MAb-30, were purified and partially characterized. MAb-11, MAb-18, and MAb-20 inhibited more than 80% of the enzyme activity and appeared to act as noncompetitive inhibitors. Competitive inhibition assay indicated that MAb-18 and MAb-20 were classified into the same group and MAb-11 into another group. MAb-20 had an inhibitory effect on acid phosphatases from some vegetable and meat sources. The Fab fragment prepared by limited proteolysis of MAb-20 with papain also inhibited the enzyme.

Original languageEnglish
Pages (from-to)1924-1928
Number of pages5
JournalBioscience, Biotechnology, and Biochemistry
Volume57
Issue number11
DOIs
Publication statusPublished - Jan 1 1993

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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