Inhibitory Monoclonal Antibody against Japanese Radish Acid Phosphatase

Ryoji Takata, Makoto Yoshimoto, Takahisa Miyamoto, Shoji Hatano, Jiro Sakamoto, Takahiko Soeda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Thirty stable hybridoma cell lines secreting monoclonal antibodies specific for Japanese radish acid phosphatase (EC 3.1.3.2) were obtained. These antibodies were inhibitory or stimulatory or had no effect on the enzyme activity. Four antibodies (IgG1 subclass) among them, designated MAb-11, MAb-18, MAb-20, and MAb-30, were purified and partially characterized. MAb-11, MAb-18, and MAb-20 inhibited more than 80% of the enzyme activity and appeared to act as noncompetitive inhibitors. Competitive inhibition assay indicated that MAb-18 and MAb-20 were classified into the same group and MAb-11 into another group. MAb-20 had an inhibitory effect on acid phosphatases from some vegetable and meat sources. The Fab fragment prepared by limited proteolysis of MAb-20 with papain also inhibited the enzyme.

Original languageEnglish
Pages (from-to)1924-1928
Number of pages5
JournalBioscience, Biotechnology, and Biochemistry
Volume57
Issue number11
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

Raphanus
Enzyme activity
Acid Phosphatase
Monoclonal Antibodies
Proteolysis
Immunoglobulin Fab Fragments
Papain
Antibodies
Meats
Vegetables
Enzymes
Assays
Immunoglobulin G
Cells
Hybridomas
Meat
Cell Line

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Inhibitory Monoclonal Antibody against Japanese Radish Acid Phosphatase. / Takata, Ryoji; Yoshimoto, Makoto; Miyamoto, Takahisa; Hatano, Shoji; Sakamoto, Jiro; Soeda, Takahiko.

In: Bioscience, Biotechnology, and Biochemistry, Vol. 57, No. 11, 01.01.1993, p. 1924-1928.

Research output: Contribution to journalArticle

Takata, Ryoji ; Yoshimoto, Makoto ; Miyamoto, Takahisa ; Hatano, Shoji ; Sakamoto, Jiro ; Soeda, Takahiko. / Inhibitory Monoclonal Antibody against Japanese Radish Acid Phosphatase. In: Bioscience, Biotechnology, and Biochemistry. 1993 ; Vol. 57, No. 11. pp. 1924-1928.
@article{20014a98c7cd4ad2bb19fbd8a47cbc47,
title = "Inhibitory Monoclonal Antibody against Japanese Radish Acid Phosphatase",
abstract = "Thirty stable hybridoma cell lines secreting monoclonal antibodies specific for Japanese radish acid phosphatase (EC 3.1.3.2) were obtained. These antibodies were inhibitory or stimulatory or had no effect on the enzyme activity. Four antibodies (IgG1 subclass) among them, designated MAb-11, MAb-18, MAb-20, and MAb-30, were purified and partially characterized. MAb-11, MAb-18, and MAb-20 inhibited more than 80{\%} of the enzyme activity and appeared to act as noncompetitive inhibitors. Competitive inhibition assay indicated that MAb-18 and MAb-20 were classified into the same group and MAb-11 into another group. MAb-20 had an inhibitory effect on acid phosphatases from some vegetable and meat sources. The Fab fragment prepared by limited proteolysis of MAb-20 with papain also inhibited the enzyme.",
author = "Ryoji Takata and Makoto Yoshimoto and Takahisa Miyamoto and Shoji Hatano and Jiro Sakamoto and Takahiko Soeda",
year = "1993",
month = "1",
day = "1",
doi = "10.1271/bbb.57.1924",
language = "English",
volume = "57",
pages = "1924--1928",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "11",

}

TY - JOUR

T1 - Inhibitory Monoclonal Antibody against Japanese Radish Acid Phosphatase

AU - Takata, Ryoji

AU - Yoshimoto, Makoto

AU - Miyamoto, Takahisa

AU - Hatano, Shoji

AU - Sakamoto, Jiro

AU - Soeda, Takahiko

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Thirty stable hybridoma cell lines secreting monoclonal antibodies specific for Japanese radish acid phosphatase (EC 3.1.3.2) were obtained. These antibodies were inhibitory or stimulatory or had no effect on the enzyme activity. Four antibodies (IgG1 subclass) among them, designated MAb-11, MAb-18, MAb-20, and MAb-30, were purified and partially characterized. MAb-11, MAb-18, and MAb-20 inhibited more than 80% of the enzyme activity and appeared to act as noncompetitive inhibitors. Competitive inhibition assay indicated that MAb-18 and MAb-20 were classified into the same group and MAb-11 into another group. MAb-20 had an inhibitory effect on acid phosphatases from some vegetable and meat sources. The Fab fragment prepared by limited proteolysis of MAb-20 with papain also inhibited the enzyme.

AB - Thirty stable hybridoma cell lines secreting monoclonal antibodies specific for Japanese radish acid phosphatase (EC 3.1.3.2) were obtained. These antibodies were inhibitory or stimulatory or had no effect on the enzyme activity. Four antibodies (IgG1 subclass) among them, designated MAb-11, MAb-18, MAb-20, and MAb-30, were purified and partially characterized. MAb-11, MAb-18, and MAb-20 inhibited more than 80% of the enzyme activity and appeared to act as noncompetitive inhibitors. Competitive inhibition assay indicated that MAb-18 and MAb-20 were classified into the same group and MAb-11 into another group. MAb-20 had an inhibitory effect on acid phosphatases from some vegetable and meat sources. The Fab fragment prepared by limited proteolysis of MAb-20 with papain also inhibited the enzyme.

UR - http://www.scopus.com/inward/record.url?scp=85007799822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007799822&partnerID=8YFLogxK

U2 - 10.1271/bbb.57.1924

DO - 10.1271/bbb.57.1924

M3 - Article

VL - 57

SP - 1924

EP - 1928

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 11

ER -