Initiation of translation at CUG, GUG, and ACG codons in mammalian cells

Haider Mehdi, Etsuro Ono, Kailash C. Gupta

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Site-directed mutants of the ACG start codon of the C′ protein encoded in the polycistronic Sendai virus P/C mRNA revealed that CUG, GUG, and ACG codons initiated translation rather efficiently (10-30% of the AUG initiation) in COS-1 host cells. In addition, AUA and AUU codons initiated translation at about 5% efficiency, while UUG did not initiate translation. The sequence context of these start codons (purine residues at -3 and +4) was crucial in their recognition by the ribosome. The location of the non-AUG codons in the P/C mRNA did not play a role in its recognition by ribosomes. By using CUG, the most efficient non-AUG start codon, instead of the original ACG codon and inserting an additional upstream CUG codon in the P/C mRNA, the amount of the C′ protein was increased and a novel protein was synthesized. Syntheses of an increased level of C′ and the novel protein did not affect downstream initiations of the P and C proteins, suggesting that more ribosomes bind the mRNA than are actually utilized for initiation of translation.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalGene
Volume91
Issue number2
DOIs
Publication statusPublished - Jul 16 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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