Injured foodborne pathogens in the vegetable production process

Currently, injury of foodborne pathogen in agricultural environment is unclear. So, we observed survival of foodborne pathogens on common procedure for growing vegetable by cultivation on two types of agar medium that possess different selectivity. In case of Salmonella enterica serovar Infantis (SI) on the process of manure preparation, injury was estimated by cultivation on selective agar medium and resuscitation on non-selective agar medium. While in Listeria monocytogenes (LM) on cultivation environment of tomato, physiological changes were confirmed by salt content in agar medium. In manure environment, insufficient elevation of temperature in piled feedstock caused injured SI. LM survived for long term and part of these survived as injured LM in soil and on the surface of tomato fruits. It might be possible to estimate injured SI in actual condition of manure preparation by above procedure. On the contrary, there are practical difficulties for discriminating injured LM in actual agricultural environment by only salt sensitivity. （Accepted Dec. 8, 2017）.