Inorganic/whole-cell biohybrid photocatalyst for highly efficient hydrogen production from water

Yuki Honda, Motonori Watanabe, Hidehisa Hagiwara, Shintaro Ida, Tatsumi Ishihara

Research output: Contribution to journalArticle

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Abstract

To obtain a clean hydrogen production system, we have developed an inorganic-bio hybrid photocatalyst system based on the combination of anatase TiO2, methylviologen (MV) as an electron mediator, and a whole-cell biocatalyst consisting of [FeFe]-hydrogenase and maturase gene-harboring recombinant Escherichia coli; however, the apparent quantum yield at 300 nm (AQY300) for hydrogen production was low (0.3%). The system consists of a two-step reaction: (1) photocatalytic MV reduction by TiO2, and (2) hydrogen production with reduced MV using a biocatalyst. The enhancement of step 1 under biocatalyst-friendly conditions was investigated in an attempt to further improve the reaction efficiency. Among the condition tested, the use of 100 mM Tris-HCl (pH 7), 150 mM NaCl, and 5% (v/v) glycerol with P-25 TiO2 especially enhanced the step 1 reaction by a 300-fold increase in the MV reduction rate compared with previously tested reaction condition (100 mM Tris-HCl (pH 7), 150 mM NaCl, 5% (v/v) glycerol, and 100 mM ascorbate with anatase TiO2). Under the enhanced step 1 reaction, AQY300 and AQY350 for photocatalytic MV reduction reached 60.8% and 52.2%, respectively. The enhanced step 1 reaction thus significantly improved the overall photocatalytic hydrogen productivity of the hybrid system and AQY300 and AQY350 reached 26.4% and 31.2%, respectively. The inorganic-whole-cell biohybrid system can therefore provide noble metal-free, efficient, and clean hydrogen production.

Original languageEnglish
Pages (from-to)400-406
Number of pages7
JournalApplied Catalysis B: Environmental
Volume210
DOIs
Publication statusPublished - Jan 1 2017

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Photocatalysts
Hydrogen production
Biocatalysts
Quantum yield
hydrogen
Water
Glycerol
Titanium dioxide
anatase
Enzymes
Hydrogenase
water
Precious metals
Hybrid systems
Escherichia coli
Hydrogen
Genes
production system
Productivity
Electrons

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Environmental Science(all)
  • Process Chemistry and Technology

Cite this

Inorganic/whole-cell biohybrid photocatalyst for highly efficient hydrogen production from water. / Honda, Yuki; Watanabe, Motonori; Hagiwara, Hidehisa; Ida, Shintaro; Ishihara, Tatsumi.

In: Applied Catalysis B: Environmental, Vol. 210, 01.01.2017, p. 400-406.

Research output: Contribution to journalArticle

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