Inositol 1,4,5-trisphosphate affinity chromatography

Masato Hirata, Yutaka Watanabe, Toyohiro Ishimatsu, Fumi Yanaga, Toshitaka Koga, Shoichiro Ozaki

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Inositol 1,4,5-trisphosphate (IP3) affinity columns were made by coupling IP3 analogs to a supporting matrix, Sepharose 4B. IP3 5-phosphatase activity, IP3 3-kinase activity and IP3 binding activity from rat brain were adsorbed on the IP3 columns, and were eluted by increasing KCl concentration. This purification procedure increased the specific activities of these parameters 5-200-fold. Thus Sepharose 4B immobilized IP3 analogs can specifically interact with IP3-binding proteins, demonstrating that IP3 affinity columns are a good method for purifying such proteins. Furthermore, our results suggest that IP3 analogs can be linked to other molecules to make useful derivatives without loss of their biological activities.

Original languageEnglish
Pages (from-to)379-386
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume168
Issue number1
DOIs
Publication statusPublished - Apr 16 1990

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hirata, M., Watanabe, Y., Ishimatsu, T., Yanaga, F., Koga, T., & Ozaki, S. (1990). Inositol 1,4,5-trisphosphate affinity chromatography. Biochemical and Biophysical Research Communications, 168(1), 379-386. https://doi.org/10.1016/0006-291X(90)91719-9