TY - JOUR
T1 - Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines
AU - Tsunoda, Toshiyuki
AU - Koga, Hirofumi
AU - Yokomizo, Akira
AU - Tatsugami, Katsunori
AU - Eto, Masatoshi
AU - Inokuchi, Junichi
AU - Hirata, Akira
AU - Masuda, Katsuaki
AU - Okumura, Koji
AU - Naito, Seiji
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (C), No. 14571506, 2002 from the Japanese Ministry of Education, Culture, Sports, Science, and Technology. We are grateful to Dr TC Sudhof, Director of the Center for Basic Neuroscience, Department of Molecular Genetics, and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, and Dr M Iino, Director of the Department of Pharmacology, Graduate School of Medicine, University of Tokyo, Japan, who donated the pCDNA3-IP3R1 mammalian expression vector. We also thank M Matsuda for expert advice; N Hakoda, M Yamada, T Kuramachi, and H Matoba for technical assistance; and B Quinn and KM Rudiger for help in preparing the manuscript.
PY - 2005/2/17
Y1 - 2005/2/17
N2 - To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.
AB - To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.
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U2 - 10.1038/sj.onc.1208313
DO - 10.1038/sj.onc.1208313
M3 - Article
C2 - 15608674
AN - SCOPUS:20044391606
VL - 24
SP - 1396
EP - 1402
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 8
ER -
