Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines

Toshiyuki Tsunoda, Hirofumi Koga, Akira Yokomizo, Katsunori Tatsugami, Masatoshi Eto, Junichi Inokuchi, Akira Hirata, Katsuaki Masuda, Koji Okumura, Seiji Naito

Research output: Contribution to journalArticle

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Abstract

To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.

Original languageEnglish
Pages (from-to)1396-1402
Number of pages7
JournalOncogene
Volume24
Issue number8
DOIs
Publication statusPublished - Feb 17 2005

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Inositol 1,4,5-Trisphosphate Receptors
Inositol 1,4,5-Trisphosphate
Urinary Bladder Neoplasms
Cisplatin
Cell Line
Apoptosis
Oligonucleotide Array Sequence Analysis
Endoplasmic Reticulum
Small Interfering RNA
Membrane Proteins
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

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Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines. / Tsunoda, Toshiyuki; Koga, Hirofumi; Yokomizo, Akira; Tatsugami, Katsunori; Eto, Masatoshi; Inokuchi, Junichi; Hirata, Akira; Masuda, Katsuaki; Okumura, Koji; Naito, Seiji.

In: Oncogene, Vol. 24, No. 8, 17.02.2005, p. 1396-1402.

Research output: Contribution to journalArticle

Tsunoda, Toshiyuki ; Koga, Hirofumi ; Yokomizo, Akira ; Tatsugami, Katsunori ; Eto, Masatoshi ; Inokuchi, Junichi ; Hirata, Akira ; Masuda, Katsuaki ; Okumura, Koji ; Naito, Seiji. / Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines. In: Oncogene. 2005 ; Vol. 24, No. 8. pp. 1396-1402.
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