Abstract
To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1396-1402 |
| Number of pages | 7 |
| Journal | Oncogene |
| Volume | 24 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Feb 17 2005 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
- Cancer Research
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Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines. / Tsunoda, Toshiyuki; Koga, Hirofumi; Yokomizo, Akira; Tatsugami, Katsunori; Eto, Masatoshi; Inokuchi, Junichi; Hirata, Akira; Masuda, Katsuaki; Okumura, Koji; Naito, Seiji.
In: Oncogene, Vol. 24, No. 8, 17.02.2005, p. 1396-1402.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines
AU - Tsunoda, Toshiyuki
AU - Koga, Hirofumi
AU - Yokomizo, Akira
AU - Tatsugami, Katsunori
AU - Eto, Masatoshi
AU - Inokuchi, Junichi
AU - Hirata, Akira
AU - Masuda, Katsuaki
AU - Okumura, Koji
AU - Naito, Seiji
PY - 2005/2/17
Y1 - 2005/2/17
N2 - To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.
AB - To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=20044391606&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20044391606&partnerID=8YFLogxK
U2 - 10.1038/sj.onc.1208313
DO - 10.1038/sj.onc.1208313
M3 - Article
C2 - 15608674
AN - SCOPUS:20044391606
VL - 24
SP - 1396
EP - 1402
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 8
ER -