Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines

Toshiyuki Tsunoda; Hirofumi Koga; Akira Yokomizo; Katsunori Tatsugami; Masatoshi Eto; Junichi Inokuchi; Akira Hirata; Katsuaki Masuda; Koji Okumura; Seiji Naito

doi:10.1038/sj.onc.1208313

Inositol 1,4,5-trisphosphate (IP₃) receptor type1 (IP ₃R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines

Toshiyuki Tsunoda, Hirofumi Koga, Akira Yokomizo, Katsunori Tatsugami, Masatoshi Eto, Junichi Inokuchi, Akira Hirata, Katsuaki Masuda, Koji Okumura, Seiji Naito

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP₃) receptor type1 (IP₃R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP₃R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP₃R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP₃R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.

Original language	English
Pages (from-to)	1396-1402
Number of pages	7
Journal	Oncogene
Volume	24
Issue number	8
DOIs	https://doi.org/10.1038/sj.onc.1208313
Publication status	Published - Feb 17 2005

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Cancer Research

Access to Document

10.1038/sj.onc.1208313

Fingerprint Dive into the research topics of 'Inositol 1,4,5-trisphosphate (IP<sub>3</sub>) receptor type1 (IP <sub>3</sub>R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines'. Together they form a unique fingerprint.

Cite this

Inositol 1,4,5-trisphosphate (IP₃) receptor type1 (IP ₃R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines. / Tsunoda, Toshiyuki; Koga, Hirofumi; Yokomizo, Akira; Tatsugami, Katsunori; Eto, Masatoshi ; Inokuchi, Junichi; Hirata, Akira; Masuda, Katsuaki; Okumura, Koji; Naito, Seiji.

In: Oncogene, Vol. 24, No. 8, 17.02.2005, p. 1396-1402.

Research output: Contribution to journal › Article › peer-review

Tsunoda, Toshiyuki ; Koga, Hirofumi ; Yokomizo, Akira ; Tatsugami, Katsunori ; Eto, Masatoshi ; Inokuchi, Junichi ; Hirata, Akira ; Masuda, Katsuaki ; Okumura, Koji ; Naito, Seiji. / Inositol 1,4,5-trisphosphate (IP₃) receptor type1 (IP ₃R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines. In: Oncogene. 2005 ; Vol. 24, No. 8. pp. 1396-1402.

@article{d41a811497aa43658fd36088eb2c3fc1,

title = "Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines",

abstract = "To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.",

author = "Toshiyuki Tsunoda and Hirofumi Koga and Akira Yokomizo and Katsunori Tatsugami and Masatoshi Eto and Junichi Inokuchi and Akira Hirata and Katsuaki Masuda and Koji Okumura and Seiji Naito",

year = "2005",

month = feb,

day = "17",

doi = "10.1038/sj.onc.1208313",

language = "English",

volume = "24",

pages = "1396--1402",

journal = "Oncogene",

issn = "0950-9232",

publisher = "Nature Publishing Group",

number = "8",

}

TY - JOUR

T1 - Inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP 3R1) modulates the acquisition of cisplatin resistance in bladder cancer cell lines

AU - Tsunoda, Toshiyuki

AU - Koga, Hirofumi

AU - Yokomizo, Akira

AU - Tatsugami, Katsunori

AU - Eto, Masatoshi

AU - Inokuchi, Junichi

AU - Hirata, Akira

AU - Masuda, Katsuaki

AU - Okumura, Koji

AU - Naito, Seiji

PY - 2005/2/17

Y1 - 2005/2/17

N2 - To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.

AB - To investigate the molecules that regulate the acquisition of cis-diamminedichloroplatinum (II) (cisplatin) resistance, we performed cDNA microarrays using two pairs of parental and cisplatin-resistant bladder cancer cell lines. We found a markedly reduced expression of inositol 1,4,5-trisphosphate (IP3) receptor type1 (IP3R1), endoplasmic reticulum membrane protein, in cisplatin-resistant cells. The suppression of IP3R1 expression using small interfering RNA in parental cells prevented apoptosis and resulted in decreased sensitivity to cisplatin. Contrarily, overexpression of IP3R1 in resistant cells induced apoptosis and increased sensitivity to cisplatin. These results suggest that cisplatin-induced downregulation of IP3R1 expression was closely associated with the acquisition of cisplatin resistance in bladder cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=20044391606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20044391606&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1208313

DO - 10.1038/sj.onc.1208313

M3 - Article

C2 - 15608674

AN - SCOPUS:20044391606

VL - 24

SP - 1396

EP - 1402

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 8

ER -