Inositol trisphosphate/Ca2+ as messengers of bradykinin B2 and muscarinic acetylcholine m1-m4 receptors in neuroblastoma-derived hybrid cells

Mami Noda, Nobuto Ishizaka, Shigeru Yokoyama, Naoto Hoshi, Yasuhiro Kimura, Minako Hashii, Megumi Taketo, Alla Egorova, Rimma Knijnik, Kazuhiko Fukuda, Hitoshi Morikawa, David A. Brown, Haruhiro Higashida

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Abstract

Neuroblastoma x glioma hybrid NG108-15 and neuroblastoma x fibroblast hybrid NL308 cells possess endogenous bradykinin B2 receptors and m4 muscarinic acetylcholine receptors (mAChRs), which couple to phospholipase C and adenylate cyclase, respectively. Four genetic subtypes of mAChRs differed in their effects when stimulated in NG108-15 and NL308 cells overexpressing mAChRs. Broadly speaking, the principal effects fell into two categories: the odd-numbered receptors (m1 and m3) activated phospholipase C and increased inositol trisphosphate/Ca2+, as bradykinin did, whereas the even-numbered receptors (m2 and m4) inhibited adenylate cyclase via a pertussis toxin (PTx)-sensitive G-protein in NG108-15 cells. But all four types of NL308 cells overexpressing each m1, m2, m3 and m4 receptor activated phospholipase C, while keeping the PTx-sensitivity in m2/m4, but not in m1/m3 receptors. Coupling to ion channel effectors showed a comparable dichotomy in NG108-15 cells, while cross-activation occurred in NL308 cells.

Original languageEnglish
Pages (from-to)175-185
Number of pages11
JournalJournal of Lipid Mediators and Cell Signalling
Volume14
Issue number1-3
DOIs
Publication statusPublished - Sep 1996
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

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