Inositol trisphosphate/Ca2+ as messengers of bradykinin B2 and muscarinic acetylcholine m1-m4 receptors in neuroblastoma-derived hybrid cells

Mami Noda, Nobuto Ishizaka, Shigeru Yokoyama, Naoto Hoshi, Yasuhiro Kimura, Minako Hashii, Megumi Taketo, Alla Egorova, Rimma Knijnik, Kazuhiko Fukuda, Hitoshi Morikawa, David A. Brown, Haruhiro Higashida

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Neuroblastoma x glioma hybrid NG108-15 and neuroblastoma x fibroblast hybrid NL308 cells possess endogenous bradykinin B2 receptors and m4 muscarinic acetylcholine receptors (mAChRs), which couple to phospholipase C and adenylate cyclase, respectively. Four genetic subtypes of mAChRs differed in their effects when stimulated in NG108-15 and NL308 cells overexpressing mAChRs. Broadly speaking, the principal effects fell into two categories: the odd-numbered receptors (m1 and m3) activated phospholipase C and increased inositol trisphosphate/Ca2+, as bradykinin did, whereas the even-numbered receptors (m2 and m4) inhibited adenylate cyclase via a pertussis toxin (PTx)-sensitive G-protein in NG108-15 cells. But all four types of NL308 cells overexpressing each m1, m2, m3 and m4 receptor activated phospholipase C, while keeping the PTx-sensitivity in m2/m4, but not in m1/m3 receptors. Coupling to ion channel effectors showed a comparable dichotomy in NG108-15 cells, while cross-activation occurred in NL308 cells.

Original languageEnglish
Pages (from-to)175-185
Number of pages11
JournalJournal of Lipid Mediators and Cell Signalling
Volume14
Issue number1-3
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

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Hybrid Cells
Type C Phospholipases
Bradykinin
Muscarinic Receptors
Inositol
Neuroblastoma
Cholinergic Agents
Acetylcholine
Pertussis Toxin
Adenylyl Cyclases
Bradykinin B2 Receptors
Fibroblasts
Ion Channels
GTP-Binding Proteins
Chemical activation
Glioma

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

Cite this

Inositol trisphosphate/Ca2+ as messengers of bradykinin B2 and muscarinic acetylcholine m1-m4 receptors in neuroblastoma-derived hybrid cells. / Noda, Mami; Ishizaka, Nobuto; Yokoyama, Shigeru; Hoshi, Naoto; Kimura, Yasuhiro; Hashii, Minako; Taketo, Megumi; Egorova, Alla; Knijnik, Rimma; Fukuda, Kazuhiko; Morikawa, Hitoshi; Brown, David A.; Higashida, Haruhiro.

In: Journal of Lipid Mediators and Cell Signalling, Vol. 14, No. 1-3, 01.01.1996, p. 175-185.

Research output: Contribution to journalArticle

Noda, M, Ishizaka, N, Yokoyama, S, Hoshi, N, Kimura, Y, Hashii, M, Taketo, M, Egorova, A, Knijnik, R, Fukuda, K, Morikawa, H, Brown, DA & Higashida, H 1996, 'Inositol trisphosphate/Ca2+ as messengers of bradykinin B2 and muscarinic acetylcholine m1-m4 receptors in neuroblastoma-derived hybrid cells', Journal of Lipid Mediators and Cell Signalling, vol. 14, no. 1-3, pp. 175-185. https://doi.org/10.1016/0929-7855(96)00523-8
Noda, Mami ; Ishizaka, Nobuto ; Yokoyama, Shigeru ; Hoshi, Naoto ; Kimura, Yasuhiro ; Hashii, Minako ; Taketo, Megumi ; Egorova, Alla ; Knijnik, Rimma ; Fukuda, Kazuhiko ; Morikawa, Hitoshi ; Brown, David A. ; Higashida, Haruhiro. / Inositol trisphosphate/Ca2+ as messengers of bradykinin B2 and muscarinic acetylcholine m1-m4 receptors in neuroblastoma-derived hybrid cells. In: Journal of Lipid Mediators and Cell Signalling. 1996 ; Vol. 14, No. 1-3. pp. 175-185.
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