Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan

I. Egami, Kazuhiro Iiyama, P. Zhang, Y. Chieda, N. Ino, K. Hasegawa, Man Lee, Takahiro Kusakabe, Chisa Yasunaga-Aoki, S. Shimizu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Twenty bacteria were isolated from four ant lion larvae. The isolates were classified into three groups by biological characteristics. Since Group I, Group II and Group III were isolated from individual larvae Kuo1, Kuo3, 4 and Kuo2, respectively, with exception of one isolate Kuo2-1, each ant lion tested had its own dominant bacterial flora. Groups I and II were closer to Serratia liquefaciens and Enterobacter cloacae, respectively, whereas Group III could not be identified by the test used. The phylogenetic analysis of GroEL amino acid sequences revealed that Group I, II and III were related to those of Serratia spp., E. cloacae and Salmonella spp. -Escherichia/Shigella spp., respectively. Among these groups, Group I was highly virulent against Bombyx mori and Periplaneta americana, and caused 100% mortality within 24 h. The other two groups (Group II and III) were avirulent to these insect species. The culture filtrate of Group I caused killing activity to B. mori larvae and the insecticidal substance was purified from culture filtrate of Group I bacterium. Since the insecticidal activity highly correlated with proteolytic activity in the chromatographies, Group I bacterium may secret insecticidal proteinase in vitro.

Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalJournal of Applied Entomology
Volume133
Issue number2
DOIs
Publication statusPublished - Mar 1 2009

Fingerprint

Myrmeleontidae
Enterobacter cloacae
Serratia
culture filtrates
Japan
Bombyx mori
larvae
bacteria
Escherichia
Shigella
Periplaneta americana
Salmonella
chromatography
proteinases
amino acid sequences
flora
insects
phylogeny
testing

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Insect Science

Cite this

Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan. / Egami, I.; Iiyama, Kazuhiro; Zhang, P.; Chieda, Y.; Ino, N.; Hasegawa, K.; Lee, Man; Kusakabe, Takahiro; Yasunaga-Aoki, Chisa; Shimizu, S.

In: Journal of Applied Entomology, Vol. 133, No. 2, 01.03.2009, p. 117-124.

Research output: Contribution to journalArticle

Egami, I. ; Iiyama, Kazuhiro ; Zhang, P. ; Chieda, Y. ; Ino, N. ; Hasegawa, K. ; Lee, Man ; Kusakabe, Takahiro ; Yasunaga-Aoki, Chisa ; Shimizu, S. / Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan. In: Journal of Applied Entomology. 2009 ; Vol. 133, No. 2. pp. 117-124.
@article{0fa4652984da4c8ea09088f24e6e2b89,
title = "Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan",
abstract = "Twenty bacteria were isolated from four ant lion larvae. The isolates were classified into three groups by biological characteristics. Since Group I, Group II and Group III were isolated from individual larvae Kuo1, Kuo3, 4 and Kuo2, respectively, with exception of one isolate Kuo2-1, each ant lion tested had its own dominant bacterial flora. Groups I and II were closer to Serratia liquefaciens and Enterobacter cloacae, respectively, whereas Group III could not be identified by the test used. The phylogenetic analysis of GroEL amino acid sequences revealed that Group I, II and III were related to those of Serratia spp., E. cloacae and Salmonella spp. -Escherichia/Shigella spp., respectively. Among these groups, Group I was highly virulent against Bombyx mori and Periplaneta americana, and caused 100{\%} mortality within 24 h. The other two groups (Group II and III) were avirulent to these insect species. The culture filtrate of Group I caused killing activity to B. mori larvae and the insecticidal substance was purified from culture filtrate of Group I bacterium. Since the insecticidal activity highly correlated with proteolytic activity in the chromatographies, Group I bacterium may secret insecticidal proteinase in vitro.",
author = "I. Egami and Kazuhiro Iiyama and P. Zhang and Y. Chieda and N. Ino and K. Hasegawa and Man Lee and Takahiro Kusakabe and Chisa Yasunaga-Aoki and S. Shimizu",
year = "2009",
month = "3",
day = "1",
doi = "10.1111/j.1439-0418.2008.01329.x",
language = "English",
volume = "133",
pages = "117--124",
journal = "Journal of Applied Entomology",
issn = "0931-2048",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan

AU - Egami, I.

AU - Iiyama, Kazuhiro

AU - Zhang, P.

AU - Chieda, Y.

AU - Ino, N.

AU - Hasegawa, K.

AU - Lee, Man

AU - Kusakabe, Takahiro

AU - Yasunaga-Aoki, Chisa

AU - Shimizu, S.

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Twenty bacteria were isolated from four ant lion larvae. The isolates were classified into three groups by biological characteristics. Since Group I, Group II and Group III were isolated from individual larvae Kuo1, Kuo3, 4 and Kuo2, respectively, with exception of one isolate Kuo2-1, each ant lion tested had its own dominant bacterial flora. Groups I and II were closer to Serratia liquefaciens and Enterobacter cloacae, respectively, whereas Group III could not be identified by the test used. The phylogenetic analysis of GroEL amino acid sequences revealed that Group I, II and III were related to those of Serratia spp., E. cloacae and Salmonella spp. -Escherichia/Shigella spp., respectively. Among these groups, Group I was highly virulent against Bombyx mori and Periplaneta americana, and caused 100% mortality within 24 h. The other two groups (Group II and III) were avirulent to these insect species. The culture filtrate of Group I caused killing activity to B. mori larvae and the insecticidal substance was purified from culture filtrate of Group I bacterium. Since the insecticidal activity highly correlated with proteolytic activity in the chromatographies, Group I bacterium may secret insecticidal proteinase in vitro.

AB - Twenty bacteria were isolated from four ant lion larvae. The isolates were classified into three groups by biological characteristics. Since Group I, Group II and Group III were isolated from individual larvae Kuo1, Kuo3, 4 and Kuo2, respectively, with exception of one isolate Kuo2-1, each ant lion tested had its own dominant bacterial flora. Groups I and II were closer to Serratia liquefaciens and Enterobacter cloacae, respectively, whereas Group III could not be identified by the test used. The phylogenetic analysis of GroEL amino acid sequences revealed that Group I, II and III were related to those of Serratia spp., E. cloacae and Salmonella spp. -Escherichia/Shigella spp., respectively. Among these groups, Group I was highly virulent against Bombyx mori and Periplaneta americana, and caused 100% mortality within 24 h. The other two groups (Group II and III) were avirulent to these insect species. The culture filtrate of Group I caused killing activity to B. mori larvae and the insecticidal substance was purified from culture filtrate of Group I bacterium. Since the insecticidal activity highly correlated with proteolytic activity in the chromatographies, Group I bacterium may secret insecticidal proteinase in vitro.

UR - http://www.scopus.com/inward/record.url?scp=60449097568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60449097568&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0418.2008.01329.x

DO - 10.1111/j.1439-0418.2008.01329.x

M3 - Article

AN - SCOPUS:60449097568

VL - 133

SP - 117

EP - 124

JO - Journal of Applied Entomology

JF - Journal of Applied Entomology

SN - 0931-2048

IS - 2

ER -