Insertion and deletion mismatches distant from the target position improve gene correction with a tailed duplex

Hiroyuki Kamiya, Natsuki Nishigaki, Akihiro Ikeda, Seiya Yukawa, Yukiko Morita, Yoshimichi Nakatsu, Teruhisa Tsuzuki, Hideyoshi Harashima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A 5′-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches ∼330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment.

Original languageEnglish
Pages (from-to)379-388
Number of pages10
JournalNucleosides, Nucleotides and Nucleic Acids
Volume35
Issue number7
DOIs
Publication statusPublished - Jul 2 2016

Fingerprint

Gene Order
Genes
DNA
HeLa Cells
Plasmids
Mutation
Substitution reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Genetics

Cite this

Insertion and deletion mismatches distant from the target position improve gene correction with a tailed duplex. / Kamiya, Hiroyuki; Nishigaki, Natsuki; Ikeda, Akihiro; Yukawa, Seiya; Morita, Yukiko; Nakatsu, Yoshimichi; Tsuzuki, Teruhisa; Harashima, Hideyoshi.

In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 35, No. 7, 02.07.2016, p. 379-388.

Research output: Contribution to journalArticle

Kamiya, Hiroyuki ; Nishigaki, Natsuki ; Ikeda, Akihiro ; Yukawa, Seiya ; Morita, Yukiko ; Nakatsu, Yoshimichi ; Tsuzuki, Teruhisa ; Harashima, Hideyoshi. / Insertion and deletion mismatches distant from the target position improve gene correction with a tailed duplex. In: Nucleosides, Nucleotides and Nucleic Acids. 2016 ; Vol. 35, No. 7. pp. 379-388.
@article{cde3d6d9a03f40aa91bc6ea35b875266,
title = "Insertion and deletion mismatches distant from the target position improve gene correction with a tailed duplex",
abstract = "A 5′-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches ∼330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment.",
author = "Hiroyuki Kamiya and Natsuki Nishigaki and Akihiro Ikeda and Seiya Yukawa and Yukiko Morita and Yoshimichi Nakatsu and Teruhisa Tsuzuki and Hideyoshi Harashima",
year = "2016",
month = "7",
day = "2",
doi = "10.1080/15257770.2016.1163384",
language = "English",
volume = "35",
pages = "379--388",
journal = "Nucleosides, Nucleotides and Nucleic Acids",
issn = "1525-7770",
number = "7",

}

TY - JOUR

T1 - Insertion and deletion mismatches distant from the target position improve gene correction with a tailed duplex

AU - Kamiya, Hiroyuki

AU - Nishigaki, Natsuki

AU - Ikeda, Akihiro

AU - Yukawa, Seiya

AU - Morita, Yukiko

AU - Nakatsu, Yoshimichi

AU - Tsuzuki, Teruhisa

AU - Harashima, Hideyoshi

PY - 2016/7/2

Y1 - 2016/7/2

N2 - A 5′-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches ∼330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment.

AB - A 5′-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches ∼330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment.

UR - http://www.scopus.com/inward/record.url?scp=84973131579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973131579&partnerID=8YFLogxK

U2 - 10.1080/15257770.2016.1163384

DO - 10.1080/15257770.2016.1163384

M3 - Article

C2 - 27253876

AN - SCOPUS:84973131579

VL - 35

SP - 379

EP - 388

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

SN - 1525-7770

IS - 7

ER -